1887

Abstract

Five Gram-negative, white-pigmented, spherical, chemoheterotrophic bacteria were isolated from seawater from Japan and the Republic of Palau by use of an cultivation technique. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the five novel isolates, 02PA-Ca-133, YM14-201, H-MN57, H-MN48 and MN1-156, were closely affiliated to members of subdivision 4 within the phylum ‘’. The novel isolates shared 96–100 % sequence similarity with each other and showed less than 90 % similarity with the cultivated strains of subdivision 4. DNA–DNA relatedness values between strains 02PA-Ca-133, YM14-201 and H-MN57 were less than 70 %; the value commonly accepted as the threshold for the phylogenetic definition of a species. Antibiotic susceptibility tests and amino acid analysis of cell-wall hydrolysates indicated that the novel isolates did not contain muramic acid or diaminopimelic acid in their cell walls, suggesting that these strains lack peptidoglycan. The DNA G+C contents of the five strains were 51–57 mol%. The major menaquinone was MK-7 and C, C 7 and anteiso-C were the major fatty acids. On the basis of polyphasic taxonomic evidence, it is concluded that these strains should be classified as representing a new genus and three novel species in subdivision 4 of the phylum ‘’, for which the names gen. nov., sp. nov. [type strain 02PA-Ca-133 (=MBIC08004=IAM 15422=KCTC 13126)], sp. nov. [type strain YM14-201 (=MBIC08272=IAM 15421=KCTC 13124)] and sp. nov. [type strain H-MN57 (=MBIC08273=IAM 15423=KCTC 13125)] are proposed.

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2007-07-01
2020-01-27
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