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Abstract

A moderately halophilic bacterium, strain YIM 70202, was isolated from a desert soil sample collected from Egypt and was subjected to a taxonomic investigation. In a phylogenetic dendrogram based on 16S rRNA gene sequence analysis, strain YIM 70202 was affiliated to the clade, showing 94.5–96.8 % 16S rRNA gene sequence similarity to the recognized species of the genus , in which CCM 3516 was the nearest neighbour. The DNA–DNA relatedness value of the novel isolate with CCM 3516 was 12.7 %. The novel isolate grew at temperatures between 4 and 45 °C and at pH values ranging from 7.0 to 11.0, with an optimum of 30 °C and pH 8.0–9.0, respectively. Strain YIM 70202 grew optimally in the presence of 10 % NaCl (w/v) and growth was observed at NaCl concentrations in the range 1–25 % (w/v). Chemotaxonomic data revealed that strain YIM 70202 contained MK-6 as the predominant respiratory quinone, possessed -Lys–Gly as the cell-wall peptidoglycan, had phosphatidylglycerol, diphosphatidylglycerol and an unknown glycolipid as the polar lipids and contained i-C and ai-C as the predominant fatty acids. The DNA GC content was 49.7 mol%. The biochemical and chemotaxonomic properties demonstrate that strain YIM 70202 represents a novel species of the genus . The name sp. nov. is proposed with strain YIM 70202 (=CGMCC 1.6511=KCTC 3941) as the type strain.

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2007-08-01
2020-01-20
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