1887

Abstract

A bacterial isolate obtained from soil from a municipal landfill site in India was characterized using a polyphasic taxonomic approach. The colonies of the isolate were found to be yellow and highly mucoid. Comparative analysis of the 16S rRNA gene sequence showed that this isolate constitutes a distinct phyletic line within the genus , displaying >3 % sequence divergence with respect to recognized species. The generic assignment was confirmed by chemotaxonomic data, which revealed the presence of a fatty acid profile characteristic of members of the genus and consisting of saturated, unsaturated, straight-chain and branched-chain fatty acids as well as iso-C 3-OH as hydroxylated fatty acid, and the presence of an ubiquinone with eight isoprene units (Q-8) as the predominant respiratory quinone. The genotypic and phenotypic data show that strain IMMIB APB-9 merits classification as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IMMIB APB-9 (=CCUG 53152=DSM 18482).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64966-0
2007-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/5/1131.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64966-0&mimeType=html&fmt=ahah

References

  1. Bae H.-S., Im W.-T., Lee S.-T. 2005; Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. Int J Syst Evol Microbiol 55:1155–1161 [CrossRef]
    [Google Scholar]
  2. Christensen P., Cook F. D. 1978; Lysobacter , a new genus of nonfruiting, gliding bacteria with a high base ratio. Int J Syst Bacteriol 28:367–393 [CrossRef]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  7. Gordon R. E. 1966; Some criteria for the recognition of Nocardia madurae (Vincent) Blanchard. J Gen Microbiol 45:355–364 [CrossRef]
    [Google Scholar]
  8. Gordon R. E. 1967; The taxonomy of soil bacteria. In The Ecology of Soil Bacteria pp  293–321 Edited by Gray T. R. G., Parkinson B. Liverpool: Liverpool University Press;
    [Google Scholar]
  9. Gordon R. E., Mihm J. M. 1957; A comparative study of some strains received as nocardiae. J Bacteriol 73:15–27
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  11. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  13. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–223 [CrossRef]
    [Google Scholar]
  14. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsiaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  15. Riegel P., de Briel D., Prévost G., Jehl F., Monteil H. 1994; Genomic diversity among Corynebacterium jeikeium strains and comparison with biochemical characteristics. J Clin Microbiol 32:1860–1865
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  17. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  18. Yassin A. F. 1988; Chemotaxonomische Untersuchungen zur vereinfachten Differenzierung und Identifizierung von aeroben Aktinomyzeten und Mykobakterien . Inaugural-Dissertation zur erlangung des Doktorgrades der Mathematische-Naturwissenschaftlichen, Fakultät der Rheinischen, Friedrich-Wilhelms-Universität, Bonn (in German
  19. Yassin A. F., Hupfer H. 2006; Williamsia deligens sp. nov., isolated from human blood. Int J Syst Evol Microbiol 56:193–197 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64966-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64966-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error