Description of sp. nov., a nitrogen-fixing bacterium isolated from groundwater Free

Abstract

A novel, Gram-negative bacterial strain, SUA2, isolated from groundwater, was characterized using a polyphasic approach. Cells are Gram-negative, non-spore-forming, straight to curved rods with a single polar flagellum. Strain SUA2 is oxidase- and catalase-positive and is able to fix nitrogen. Poly--hydroxybutyrate storage granules are produced. Dominant fatty acids when grown in R2A and VM ethanol media for 72 h at 37 °C are C, C 7c, C cyclo, C 3-OH, C 7c, C and C. DNA G+C content is 67.9 mol%. Phenotypic and phylogenetic data indicate that strain SUA2 is related to, but clearly differentiated from . Strain SUA2 is thus proposed as a novel species of the genus with the name sp. nov. The description of the genus is emended to include the characteristics of this novel species. The type strain of is SUA2 (=NRRL B-41660=DSM 18626=LMG 23819).

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2007-07-01
2024-03-28
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References

  1. Achenbach L. A., Michaelidou U., Bruce R. A., Fryman J., Coates J. D. 2001; Dechloromonas agitata gen. nov., sp. nov. and Dechlorosoma suillum gen. nov., sp. nov., two novel environmentally dominant (per)chlorate-reducing bacteria and their phylogenetic position. Int J Syst Evol Microbiol 51:527–533
    [Google Scholar]
  2. Bowman K. S., Moe W. M., Rash B. A., Bae H. S., Rainey F. A. 2006; Bacterial diversity of an acidic Louisiana groundwater contaminated by DNAPL containing chloroethanes and other solvents. FEMS Microbiol Ecol 58:120–133 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 2004 phylip (phylogeny inference package), version 3.62. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  4. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  5. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. New York: Academic Press;
    [Google Scholar]
  6. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinformat 5:150–163 [CrossRef]
    [Google Scholar]
  7. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  8. Poly F., Monrozier L. J., Bally R. 2001; Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil. Res Microbiol 152:95–103 [CrossRef]
    [Google Scholar]
  9. Rainey F. A., Ward-Rainey N., Kroppenstedt E., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  10. Reinhold B., Hurek T., Niemann E.-G., Fendrik I. 1986; Close association of Azospirillum and diazotrophic rods with different root zones of Kallar grass. Appl Environ Microbiol 52:520–526
    [Google Scholar]
  11. Reinhold-Hurek B., Hurek T. 2000; Reassessment of the taxonomic structure of the diazotrophic genus Azoarcus sensu lato and description of three new genera and new species, Azovibrio restrictus gen.nov., sp. nov., Azospira oryzae gen. nov., sp. nov. and Azonexus fungiphilus gen. nov., sp. nov. Int J Syst Evol Microbiol 50:649–659 [CrossRef]
    [Google Scholar]
  12. Reinhold-Hurek B., Hurek T., Gillis M., Hoste B., Vancanneyt M., Kersters K., De Ley J. 1993; Azoarcus gen. nov., nitrogen-fixing proteobacteria associated with roots of Kallar grass ( Leptochlora fusca (L.) Kunth), and description of two species, Azoarcus indigens sp.nov. and Azoarcus communis sp. nov. Int J Syst Evol Microbiol 43:574–584
    [Google Scholar]
  13. Sasser M. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. MIDI Technical Note, 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  14. Simpson J. M., Santo Domingo J. W., Reasoner D. J. 2004; Assessment of equine fecal contamination: the search for alternative bacterial source-tracking targets. FEMS Microbiol Ecol 47:65–75 [CrossRef]
    [Google Scholar]
  15. Smibert R. M., Krieg N. R. 1981; General characterization. In Manual of Methods for General Bacteriology pp  409–443 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  16. Tan Z., Reinhold-Hurek B. 2003; Dechlorosoma suillum Achenbach et al. 2001 is a later subjective synonym of Azospira oryzae Reinhold-Hurek and Hurek 2000. Int J Syst Evol Microbiol 53:1139–1142 [CrossRef]
    [Google Scholar]
  17. Tarrand J. J., Gröschel D. H. M. 1982; Rapid, modified oxidase test for oxidase-variable bacterial isolates. J Clin Microbiol 16:772–774
    [Google Scholar]
  18. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
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