1887

Abstract

A novel aerobic, psychrotolerant marine bacterium was isolated at 4 °C from seawater samples collected from Spitzbergen in the Arctic. The strain was a polar-flagellated, Gram-negative bacterium that grew optimally at 10–15 °C and pH 7–8 in media containing 2–3 % NaCl (w/v), using various carbohydrates and organic acids as substrates. The main fatty acid components included 16 : 0 (12.7 % of total fatty acids), straight-chain saturated fatty acid methyl ester (FAME) and 16 : 17 (40.2 %) monounsaturated FAME. Phylogenetic analysis revealed a close relationship (99 % 16S rRNA gene sequence similarity) between the novel isolate and KMM 162 and some other species of the genus . The DNA G+C content of the novel strain was 39 mol%. DNA–DNA hybridization showed only 47.6 % DNA–DNA relatedness with KMM 162, 44.2 % with KMM 638 and 22.6 % with NCIMB 8614. Based on phylogenetic and phenotypic characteristics, this isolate represents a novel species of the genus for which the name is proposed; the type strain is A 37-1-2 (=LMG 23753=DSM 18437).

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2008-09-01
2020-01-22
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