1887

Abstract

The interrelationships of 27 strains were investigated using sequences and DNA–DNA hybridization. sequence similarities showed a stronger relationship with DNA–DNA relatedness values than did 16S rRNA gene sequence similarities. Additionally, sequence analysis, with interspecies divergence over 5.2 % in most cases, gave better resolution than 16S rRNA gene sequences for the differentiation of strains at the species level. Relationships among species were therefore elucidated on the basis of sequences and DNA–DNA reassociation. Strains of and sp. HG11 were unquestionably grouped in the same genetic species, since they shared 98.7 % sequence similarity and 82–85 % genomic relatedness. The phylogenetically close relationships obtained from sequence analysis (1.7–3.3 % genetic distance) were corroborated by high DNA–DNA relatedness (73–97 %) to support the previous suggestion that and are later heterotypic synonyms of . Our findings will contribute to the clarification of controversial relationships in the genus and also demonstrate that analysis of sequences can be a powerful tool for interspecies study of the genus.

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2007-06-01
2019-12-07
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