1887

Abstract

A single strain (designated Khorat-H2) was isolated from the urine of an adult male patient with suspected leptospirosis from the province of Nakornrachasima, Thailand. The isolate showed typical motility and morphology under dark-field microscopy. Cells were 10–13 μm long and 0.2 μm in diameter, with a wavelength of 0.5 μm and an amplitude of approximately 0.3 μm. Phenotypically, strain Khorat-H2 did not grow at 13 °C but grew at 30 and 37 °C and in the presence of 8-azaguanine. Serological identification using the microscopic agglutination test revealed that strain Khorat-H2 had no cross-reaction with any recognized serogroups. Phylogenetic analysis of the 16S rRNA gene sequence placed the novel strain within the radiation of the genus , with sequence similarities of 88.1–97.7 % to recognized species. DNA–DNA hybridization against the type strains of the three most closely related species was used to confirm the results of the 16S rRNA sequence analysis. The G+C content of strain Khorat-H2 was 41.8 mol%. On the basis of phenotypic, serological and phylogenetic data, strain Khorat-H2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Khorat-H2 (=WHO LT1686 =KIT Khorat-H2).

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2008-10-01
2019-10-17
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References

  1. Bharti, A. R., Nally, J. E., Ricaldi, J. N., Matthias, M. A., Diaz, M. M., Lovett, M. A., Levett, P. N., Gilman, R. H., Willig, M. R. & other authors ( 2003; ). Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis 3, 757–771.[CrossRef]
    [Google Scholar]
  2. Brenner, D. J., McWhorter, A. C., Knutson, J. K. & Steigerwalt, A. G. ( 1982; ). Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol 15, 1133–1140.
    [Google Scholar]
  3. Brenner, D. J., Kaufmann, A. F., Sulzer, K. R., Steigerwalt, A. G., Rogers, F. C. & Weyant, R. S. ( 1999; ). Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies. Int J Syst Bacteriol 49, 839–858.[CrossRef]
    [Google Scholar]
  4. Faine, S., Adler, B. & Bolin, C. (editors) ( 1999; ). Leptospira and Leptospirosis, 2nd edn. Armadale, Victoria: MediSci.
  5. Johnson, R. C. & Rogers, P. ( 1964; ). Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. J Bacteriol 88, 1618–1623.
    [Google Scholar]
  6. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  7. Kotilainen, P., Jalava, J., Meurman, O., Lehtonen, O. P., Rintala, E., Seppala, O. P., Eerola, E. & Nikkari, S. ( 1998; ). Diagnosis of meningococcal meningitis by broad-range bacterial PCR with cerebrospinal fluid. J Clin Microbiol 36, 2205–2209.
    [Google Scholar]
  8. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  9. Levett, P. N. ( 2001; ). Leptospirosis. Clin Microbiol Rev 14, 296–326.[CrossRef]
    [Google Scholar]
  10. Levett, P. N., Morey, R. E., Galloway, R. L. & Steigerwalt, A. G. ( 2006; ). Leptospira broomii sp. nov., isolated from humans with leptospirosis. Int J Syst Evol Microbiol 56, 671–673.[CrossRef]
    [Google Scholar]
  11. Mandel, M., Igambi, L., Bergendahl, J., Dodson, M. L., Jr & Scheltgen, E. ( 1970; ). Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. J Bacteriol 101, 333–338.
    [Google Scholar]
  12. Perolat, P., Chappel, R. J., Adler, B., Baranton, G., Bulach, D. M., Billinghurst, M. L., Letocart, M., Merien, F. & Serrano, M. S. ( 1998; ). Leptospira fainei sp. nov., isolated from pigs in Australia. Int J Syst Bacteriol 48, 851–858.[CrossRef]
    [Google Scholar]
  13. Relman, D. A., Loutit, J. S., Schmidt, T. M., Falkow, S. & Tompkins, L. S. ( 1990; ). The agent of bacillary angiomatosis. An approach to the identification of uncultured pathogens. N Engl J Med 323, 1573–1580.[CrossRef]
    [Google Scholar]
  14. Relman, D. A., Schmidt, T. M., MacDermott, R. P. & Falkow, S. ( 1992; ). Identification of the uncultured bacillus of Whipple's disease. N Engl J Med 327, 293–301.[CrossRef]
    [Google Scholar]
  15. Slack, A. T., Symonds, M. L., Dohnt, M. F. & Smythe, L. D. ( 2006; ). Identification of pathogenic Leptospira species by conventional or real-time PCR and sequencing of the DNA gyrase subunit B encoding gene. BMC Microbiol 6, 95 [CrossRef]
    [Google Scholar]
  16. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  17. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  18. WHO ( 1999; ). Leptospirosis worldwide, 1999. Wkly Epidemiol Rec 74, 237–242.
    [Google Scholar]
  19. Wilbrink, B., van der Heijden, I. M., Schouls, L. M., van Embden, J. D., Hazes, J. M., Breedveld, F. C. & Tak, P. P. ( 1998; ). Detection of bacterial DNA in joint samples from patients with undifferentiated arthritis and reactive arthritis, using polymerase chain reaction with universal 16S ribosomal RNA primers. Arthritis Rheum 41, 535–543.[CrossRef]
    [Google Scholar]
  20. Yasuda, P. H., Steigerwalt, A. G., Sulzer, C. R., Kaufmann, A. F., Rogers, F. C. & Brenner, D. J. ( 1987; ). Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species. Int J Syst Bacteriol 37, 407–415.[CrossRef]
    [Google Scholar]
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