1887

Abstract

A Gram-positive, spore-forming and moderately halophilic bacterium was isolated from fermented fish (pla-ra) in Thailand. Cells of the isolate, RBU1-1, were strictly aerobic, motile rods and contained -diaminopimelic acid in the cell-wall peptidoglycan. Menaquinone with seven isoprene units (MK-7) was the predominant quinone. This isolate grew at 15–48 °C, pH 5–9 and in 2–30 % NaCl (optimally 10–20 %). The major cellular fatty acids were iso-C and anteiso-C. Polar lipid analysis revealed the presence of phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 36.7 mol%. 16S rRNA gene sequence analysis revealed that strain RBU1-1 was a member of the family , and belonged to a cluster with and ; strain RBU1-1 showed 16S rRNA gene sequence similarities of 96.0–96.9 % to members of these two genera. Strain RBU1-1 could also be differentiated from members of the genera and based on certain phenotypic characteristics such as cell-wall composition, mode of flagellation and growth pH range. Therefore, strain RBU1-1 is considered to represent a novel species in a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is RBU1-1 (=JCM 13188=PCU 270=TISTR 1571).

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2007-07-01
2024-03-28
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References

  1. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  4. Forbes L. 1981; Rapid flagella stain. J Clin Microbiol 13:807–809
    [Google Scholar]
  5. Fritze D. 1996; Bacillus haloalkalophilus sp. nov. Int J Syst Bacteriol 46:98–101 [CrossRef]
    [Google Scholar]
  6. García M. T., Gallego V., Ventosa V., Mellado E. 2005; Thalassobacillus devorans gen. nov., sp. nov. a moderately halophilic, phenol-degrading, Gram-positive bacterium. Int J Syst Evol Microbiol 55:1789–1795 [CrossRef]
    [Google Scholar]
  7. Heyndrickx M., Lebbe L., Kersters K., Hoste B., De Wachter R., De Vos P., Forsyth G., Logan N. A. 1999; Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus panthothenticus (Proom and Knight 1950) Heyndrickx et al . 1998. Int J Syst Bacteriol 49:1083–1090 [CrossRef]
    [Google Scholar]
  8. Hucker G. J., Conn H. J. 1923; Method of gram staining. Tech Bull N Y St Agric Exp Sta 93:3–37
    [Google Scholar]
  9. Ishikawa M., Ishizaki S., Yamamoto Y., Yamasato K. 2002 Paraliobacillus ryukyuensis gen. nov., sp. nov., a new Gram-positive, slightly halophilic, extremely halotolerant, facultative anaerobe isolated from a decomposing marine alga. J Gen Appl Microbiol 48269–279 [CrossRef]
  10. Jeon C. O., Lim J.-M., Lee J.-M., Xu L.-H., Jiang C.-L., Kim C.-J. 2005; Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55:1891–1896 [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  12. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  13. Kumar S., Tamura K., Jakobson I.-B., Nei M. 2001; mega2: molecular evolutionary genetic analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  14. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  15. Lim J.-M., Jeon C. O., Sung S. M., Kim C.-J. 2005; Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 55:165–170 [CrossRef]
    [Google Scholar]
  16. Lu J., Nogi Y., Takami H. 2001; Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett 205:291–297 [CrossRef]
    [Google Scholar]
  17. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  18. Namwong S., Tanasupawat S., Smitinont T., Visessanguan W., Kudo T., Itoh T. 2005; Isolation of Lentibacillus salicampi strains and Lentibacillus juripiscarius sp. nov. from fish sauce in Thailand. Int J Syst Evol Microbiol 55:315–320 [CrossRef]
    [Google Scholar]
  19. Pakdeeto A., Tanasupawat S., Thawai C., Moonmangmee S., Kudo T., Itoh T. 2007; Lentibacillus kapialis sp. nov., from fermented shrimp paste in Thailand. Int J Syst Evol Microbiol 57:364–369 [CrossRef]
    [Google Scholar]
  20. Phithakpol B., Varanyanond W., Reungmaneepaitoon S., Wood H. 1995 The Traditional Fermented Foods of Thailand Kuala Lumpur: ASEAN Food Handling Bureau Level;3
    [Google Scholar]
  21. Ren P.-G., Zhou P.-J. 2005a; Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China. Int J Syst Evol Microbiol 55:95–99 [CrossRef]
    [Google Scholar]
  22. Ren P.-G., Zhou P.-J. 2005b Salinibacillus aidingensis gen. nov., sp. nov., and Salinibacillus kushneri sp. nov., moderately halophilic bacteria isolated from a neutral saline lake in Xin-Jiang, China. Int J Syst Evol Microbiol 55949–953 [CrossRef]
  23. Romano I., Lama L., Nicolaus B., Gambacorta A., Giordano A. 2005; Alkalibacillus filiformis sp. nov., isolated from a mineral pool in Campania, Italy. Int J Syst Evol Microbiol 55:2395–2399 [CrossRef]
    [Google Scholar]
  24. Saito H., Miura K. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629 [CrossRef]
    [Google Scholar]
  25. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  26. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . MIDI Technical Note 101: Newark, DE: MIDI Inc;
    [Google Scholar]
  27. Schlesner H., Lawson P. A., Collins M. D., Weiss N., Wehmeyer U., Völker H., Thomm M. 2001; Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51:425–431
    [Google Scholar]
  28. Spring S., Ludwig W., Marquez M. C., Ventosa A., Schleifer K.-H. 1996; Halobacillus gen. nov., with description of Halobacillus litoralis sp.nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophila comb. nov. Int J Syst Bacteriol 46:492–496 [CrossRef]
    [Google Scholar]
  29. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  30. Tanasupawat S., Shida O., Okada S., Komagata K. 2000; Lactobacillus acidipiscis sp. nov., and Weissella thailandensis sp. nov., isolated from fermented fish in Thailand. Int J Syst Evol Microbiol 50:1479–1485 [CrossRef]
    [Google Scholar]
  31. Tanasupawat S., Thawai C., Yukphan P., Moonmangmee D., Itoh T., Adachi O., Yamada Y. 2004; Gluconobacter thailandicus sp. nov., an acetic acid bacterium in the α -proteobacteria. J Gen Appl Microbiol 50:159–167 [CrossRef]
    [Google Scholar]
  32. Tanasupawat S., Pakdeeto A., Namwong S., Thawai C., Kudo T., Itoh T. 2006; Lentibacillus halophilus sp. nov., from fish sauce in Thailand. Int J Syst Evol Microbiol 56:1859–1863 [CrossRef]
    [Google Scholar]
  33. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  34. Thornley M. J. 1960; The differentiation of Pseudomonas from other Gram-negative bacteria on the basis of arginine metabolism. J Appl Bacteriol 23:37–52 [CrossRef]
    [Google Scholar]
  35. Ventosa A., Garcia M. T., Kamekura M., Onishi H., Ruiz-Berraquero M. 1989; Bacillus halophilus sp. nov., a moderately halophilic bacillus species. Syst Appl Microbiol 12:162–165 [CrossRef]
    [Google Scholar]
  36. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  37. Yoon J.-H., Kang K. H., Park Y.-H. 2002 Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. Int J Syst Evol Microbiol 522043–2048 [CrossRef]
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