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Abstract

A Gram-positive, spore-forming and moderately halophilic bacterium was isolated from fermented fish (pla-ra) in Thailand. Cells of the isolate, RBU1-1, were strictly aerobic, motile rods and contained -diaminopimelic acid in the cell-wall peptidoglycan. Menaquinone with seven isoprene units (MK-7) was the predominant quinone. This isolate grew at 15–48 °C, pH 5–9 and in 2–30 % NaCl (optimally 10–20 %). The major cellular fatty acids were iso-C and anteiso-C. Polar lipid analysis revealed the presence of phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 36.7 mol%. 16S rRNA gene sequence analysis revealed that strain RBU1-1 was a member of the family , and belonged to a cluster with and ; strain RBU1-1 showed 16S rRNA gene sequence similarities of 96.0–96.9 % to members of these two genera. Strain RBU1-1 could also be differentiated from members of the genera and based on certain phenotypic characteristics such as cell-wall composition, mode of flagellation and growth pH range. Therefore, strain RBU1-1 is considered to represent a novel species in a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is RBU1-1 (=JCM 13188=PCU 270=TISTR 1571).

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2007-07-01
2020-01-25
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vol. , part 7, pp. 1413 - 1417

Polar lipids of strain RBU1-1 . The TLC plate was sprayed with anisaldehyde reagent. DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol.



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