1887

Abstract

An actinomycete, strain 4776, was isolated from soil collected from Emei Mountain in Sichuan Province, China. The taxonomic status of this strain was established using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of streptomycetes. Phylogenetic analysis based on the almost complete 16S rRNA gene sequence indicated that the novel isolate belongs to the genus and consistently falls into a clade together with DSM 40098, JCM 4603, DSM 40590, DSM 40095 and DSM 40198. However, DNA–DNA relatedness and phenotypic data distinguished strain 4776 from these phylogenetically related type strains. It is therefore concluded that strain 4776 (=CGMCC 4.3504=DSM 41884) represents a novel species of the genus , for which the name sp. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64934-0
2007-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/7/1635.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64934-0&mimeType=html&fmt=ahah

References

  1. Al-Tai A., Kim B., Kim S. B., Manfio G. P., Goodfellow M. 1999; Streptomyces malaysiensis sp. nov., a new streptomycete species with rugose, ornamented spores. Int J Syst Bacteriol 49:1395–1402 [CrossRef]
    [Google Scholar]
  2. Bérdy J. 2005; Bioactive microbial metabolites. J Antibiot (Tokyo 58:1–26 [CrossRef]
    [Google Scholar]
  3. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [CrossRef]
    [Google Scholar]
  4. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp  267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  5. Dietera A., Hamm A., Fiedler H.-P., Goodfellow M., Müller W. E. G., Brun R., Beil W., Bringmann G. 2003; Pyrocoll, an antibiotic, antiparasitic and antitumor compound produced by a novel alkalophilic Streptomyces strain. J Antibiot (Tokyo 56:639–646 [CrossRef]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  7. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  9. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  10. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284 [CrossRef]
    [Google Scholar]
  11. Gu Q., Luo H., Zheng W., Liu Z., Huang Y. 2006; Pseudonocardia oroxyli sp. nov., a novel actinomycete isolated from the surface-sterilized Oroxylum indicum root. Int J Syst Evol Microbiol 56:2193–2197 [CrossRef]
    [Google Scholar]
  12. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–332 [CrossRef]
    [Google Scholar]
  13. He L., Li W., Huang Y., Wang L., Liu Z. H., Lanoot B., Vancanneyt M., Swings J. 2005; Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55:1939–1944 [CrossRef]
    [Google Scholar]
  14. Huang Y., Li W., Wang L., Lanoot B., Vancanneyt M., Rodriguez C., Liu Z., Swings J., Goodfellow M. 2004; Streptomyces glauciniger sp. nov., a novel mesophilic streptomycete isolated from soil in south China. Int J Syst Evol Microbiol 54:2085–2089 [CrossRef]
    [Google Scholar]
  15. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  16. Kämpfer P., Kroppenstedt R. M., Dott W. 1991; A numerical classification of the genera Streptomyces and Streptoverticillium using miniaturized physiological tests. J Gen Microbiol 137:1831–1891 [CrossRef]
    [Google Scholar]
  17. Kawato M., Shinobu R. 1959; On Streptomyces herbaricolor sp. nov., supplement: a single technique for microscopical observation. Mem Osaka Unit Lib Arts Educ B Nat Sci 8:114–119
    [Google Scholar]
  18. Kim S. B., Goodfellow M. 2002; Streptomyces avermitilis sp. nov., nom. rev. a taxonomic home for the avermectin-producing streptomycetes. Int J Syst Evol Microbiol 522011–2014 [CrossRef]
    [Google Scholar]
  19. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  20. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp  173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  21. Labeda D. P., Lechevalier M. P., Testa R. T. 1997; Streptomyces stramineus sp. nov., a new species of the verticillate streptomycetes. Int J Syst Bacteriol 47:747–753 [CrossRef]
    [Google Scholar]
  22. Lechevalier H. A., Lechevalier M. P. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy , Special Publication no. 6 pp  277–284 Edited by Dietz A., Thayer D. W. Arlington, VA: Society of Industrial Microbiology;
    [Google Scholar]
  23. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  24. Manfio G. P., Zakrzewska-Czerwinska J., Atalan E., Goodfellow M. 1995; Towards minimal standards for the description of Streptomyces species. Biotekhnologiia 8:228–237
    [Google Scholar]
  25. Marmur J., Doty P. 1962; Determination of base composition of deoxyribonucleic acid from its denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  26. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of isoprenoid quinones and polar-lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  27. Saintpierre D., Amir H., Pineau R., Sembiring L., Goodfellow M. 2003; Streptomyces yatensis sp. nov., a novel bioactive streptomycete isolated from a New-Caledonian ultramafic soil. Antonie van Leeuwenhoek 83:21–26 [CrossRef]
    [Google Scholar]
  28. Saitou N., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  29. Sasser M. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids . Technical Note 101: Newark, DE: Microbial ID;
    [Google Scholar]
  30. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  31. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  32. Van de Peer Y., De Wachter R. 1994; treecon for windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
  33. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Bacterial Systematics. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  34. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  35. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . In Bergey's Manual of Systematic Bacteriology vol. 4 pp  2594–2598 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  36. Xu C., Wang L., Cui Q., Huang Y., Liu Z., Zheng G., Goodfellow M. 2006; Neutrotolerant acidophilic Streptomyces species isolated from acidic soils in China: Streptomyces guanduensis sp. nov., Streptomyces paucisporeus sp. nov., Streptomyces rubidus sp.nov. and Streptomyces yanglinensis sp. nov. Int J Syst Evol Microbiol 56:1109–1115 [CrossRef]
    [Google Scholar]
  37. Zhou Z. H., Liu Z. H., Qiao Y. D., Kim S. B., Goodfellow M. 1998; Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 48:53–58 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64934-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64934-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error