RT Journal Article SR Electronic(1) A1 Nogi, Yuichi A1 Hosoya, Shoichi A1 Kato, Chiaki A1 Horikoshi, KokiYR 2007 T1 Psychromonas hadalis sp. nov., a novel piezophilic bacterium isolated from the bottom of the Japan Trench JF International Journal of Systematic and Evolutionary Microbiology, VO 57 IS 6 SP 1360 OP 1364 DO https://doi.org/10.1099/ijs.0.64933-0 PB Microbiology Society, SN 1466-5034, AB An obligately piezophilic bacterium was isolated from sediment collected from the bottom of the Japan Trench at a depth of 7542 m. The isolated strain, designated K41GT, was closely affiliated with members of the genus Psychromonas on the basis of 16S rRNA gene sequence analysis. Levels of DNA–DNA relatedness between strain K41GT and Psychromonas reference strains were significantly lower than that accepted as the phylogenetic definition of a species. The optimal temperature and pressure for growth of strain K41GT were 6 °C and 60 MPa, respectively. The DNA G+C content was 39.1 mol%. Whole-cell fatty acids consisted of significant amounts of unsaturated fatty acids C16 : 1 (37 %) and C14 : 1 (17 %), saturated fatty acid C16 : 0 (31 %) and polyunsaturated fatty acid C22 : 6 (8 %). Based on the taxonomic differences observed, strain K41GT is considered to represent a novel obligately piezophilic Psychromonas species. The name Psychromonas hadalis (type strain, K41GT=JCM 11830T=ATCC BAA-638T) is proposed. This is the second species of obligately piezophilic bacteria to be proposed in the genus Psychromonas., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64933-0