1887

Abstract

An obligately piezophilic bacterium was isolated from sediment collected from the bottom of the Japan Trench at a depth of 7542 m. The isolated strain, designated K41G, was closely affiliated with members of the genus on the basis of 16S rRNA gene sequence analysis. Levels of DNA–DNA relatedness between strain K41G and reference strains were significantly lower than that accepted as the phylogenetic definition of a species. The optimal temperature and pressure for growth of strain K41G were 6 °C and 60 MPa, respectively. The DNA G+C content was 39.1 mol%. Whole-cell fatty acids consisted of significant amounts of unsaturated fatty acids C (37 %) and C (17 %), saturated fatty acid C (31 %) and polyunsaturated fatty acid C (8 %). Based on the taxonomic differences observed, strain K41G is considered to represent a novel obligately piezophilic species. The name (type strain, K41G=JCM 11830=ATCC BAA-638) is proposed. This is the second species of obligately piezophilic bacteria to be proposed in the genus .

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2007-06-01
2024-12-02
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References

  1. Auman A. J., Breezee J. L., Gosink J. J., Kämpfer P., Staley J. T. 2006; Psychromonas ingrahamii sp. nov., a novel gas vacuolate, psychrophilic bacterium isolated from Arctic polar sea ice. Int J Syst Evol Microbiol 56:1001–1007 [CrossRef]
    [Google Scholar]
  2. DeLong E. F., Franks D. G., Yayanos A. A. 1997; Evolutionary relationships of cultivated psychrophilic and barophilic deep-sea bacteria. Appl Environ Microbiol 63:2105–2108
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Groudieva T., Grote R., Antranikian G. 2003; Psychromonas arctica sp. nov., a novel psychrotolerant, biofilm-forming bacterium isolated from Spitzbergen. Int J Syst Evol Microbiol 53:539–545 [CrossRef]
    [Google Scholar]
  5. Kato C., Sato T., Smorawinska M., Horikoshi K. 1994; High pressure conditions stimulate expression of chloramphenicol acetyltransferase regulated by the lac promoter in Escherichia coli . FEMS Microbiol Lett 122:91–96 [CrossRef]
    [Google Scholar]
  6. Kato C., Sato T., Horikoshi K. 1995; Isolation and properties of barophilic and barotolerant bacteria from deep-sea mud samples. Biodivers Conserv 4:1–9 [CrossRef]
    [Google Scholar]
  7. Kato C., Li L., Nogi Y., Nakamura Y., Tamaoka J., Horikoshi K. 1998; Extremely barophilic bacteria isolated from the Mariana Trench, Challenger Deep, at a depth of 11,000 meters. Appl Environ Microbiol 64:1510–1513
    [Google Scholar]
  8. Kawasaki K., Nogi Y., Hishinuma M., Nodasaka Y., Matsuyama H., Yumoto I. 2002; Psychromonas marina sp. nov., a novel halophilic, facultatively psychrophilic bacterium isolated from the coast of the Okhotsk Sea. Int J Syst Evol Microbiol 52:1455–1459 [CrossRef]
    [Google Scholar]
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  10. Margesin R., Nogi Y. 2004; Psychropiezophilic microorganisms. Cell Mol Biol 50:429–436
    [Google Scholar]
  11. Mountfort D. O., Rainey F. A., Burghardt J., Kasper F., Stackebrandt E. 1998; Psychromonas antarcticus gen. nov., sp. nov. a new aerotolerant anaerobic, halophilic psychrophile isolated from pond sediment of the McMurdo Ice Shelf, Antarctica. Arch Microbiol 169:231–238 [CrossRef]
    [Google Scholar]
  12. Nogi Y., Kato C. 1999; Taxonomic studies of extremely barophilic bacteria isolated from the Mariana Trench and description of Moritella yayanosii sp. nov., a new barophilic bacterial isolate. Extremophiles 3:71–77 [CrossRef]
    [Google Scholar]
  13. Nogi Y., Kato C., Horikoshi K. 1998; Taxonomic studies of deep-sea barophilic Shewanella strains and description of Shewanella violacea sp. nov. Arch Microbiol 170:331–338 [CrossRef]
    [Google Scholar]
  14. Nogi Y., Kato C., Horikoshi K. 2002; Psychromonas kaikoae sp. nov., a novel piezophilic bacterium from the deepest cold-seep sediments in the Japan Trench. Int J Syst Evol Microbiol 52:1527–1532 [CrossRef]
    [Google Scholar]
  15. Nogi Y., Hosoya S., Kato C., Horikoshi K. 2004; Colwellia piezophila sp. nov., a novel piezophilic species from deep-sea sediments of the Japan Trench. Int J Syst Evol Microbiol 54:1627–1631 [CrossRef]
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  17. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  18. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A. D., Kämpfer P., Maiden M. C. J., Nesme X., Rosselló-Mora R., Swings J. other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  19. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  20. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  21. Xu Y., Nogi Y., Kato C., Liang Z., Rüger H.-J., Kegel D. D., Glansdorff N. 2003; Psychromonas profunda sp. nov., a psychropiezophilic bacterium from deep Atlantic sediments. Int J Syst Evol Microbiol 53:527–532 [CrossRef]
    [Google Scholar]
  22. Yanagibayashi M., Nogi Y., Li L., Kato C. 1999; Changes in the microbial community in Japan Trench sediment from a depth of 6,292 m during cultivation without decompression. FEMS Microbiol Lett 170:271–279 [CrossRef]
    [Google Scholar]
  23. Yayanos A. A., Dietz A. S., Van Boxtel R. 1979; Isolation of a deep-sea barophilic bacterium and some of its growth characteristics. Science 205:808–810 [CrossRef]
    [Google Scholar]
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