1887

Abstract

An anaerobic, mesophilic, syntrophic, propionate-oxidizing bacterium, strain MGP, was isolated as a defined co-culture with from the methanogenic sludge of a mesophilic upflow anaerobic sludge blanket (UASB) reactor. The strain grew in the presence of propionate, but only in co-culture with methanogens, suggesting that it is an obligately syntrophic bacterium. The optimum temperature for growth was 37 °C, and the optimum pH was between 6.5 and 7.2. Based on comparative 16S rRNA gene sequence analysis, strain MGP was affiliated with subcluster Ih of ‘ cluster I’, in which it was found to be moderately related to known species of the genera and . Similar to known species of the genus , strain MGP could degrade propionate in syntrophy, but had no ability to reduce sulfate, sulfite and thiosulfate. Further phenotypic and genetic studies supported the affiliation of the strain as a novel species in this genus, for which the name sp. nov. is proposed. The type strain is MGP (=DSM 15578=JCM 11929). The strain has been deposited in the DSM and JCM culture collections as a defined co-culture with .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64925-0
2007-07-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/7/1487.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64925-0&mimeType=html&fmt=ahah

References

  1. Boone, D. R. & Bryant, M. P. ( 1980; ). Propionate-degrading bacterium, Syntrophobacter wolinii sp. nov., gen. nov., from methanogenic ecosystems. Appl Environ Microbiol 40, 626–632.
    [Google Scholar]
  2. Chen, S., Liu, X. & Dong, X. ( 2005; ). Syntrophobacter sulfatireducens sp. nov., a novel syntrophic, propionate-oxidizing bacterium isolated from UASB reactors. Int J Syst Evol Microbiol 55, 1319–1324.[CrossRef]
    [Google Scholar]
  3. de Bok, F. A. M., Harmsen, H. J. M., Plugge, C. M., de Vries, M. C., Akkermans, A. D. L., de Vos, W. M. & Stams, A. J. M. ( 2005; ). The first true obligate syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp. nov., co-cultured with Methanospirillum hungatei, and emended description of the genus Pelotomaculum. Int J Syst Evol Microbiol 55, 1697–1703.[CrossRef]
    [Google Scholar]
  4. Doetsch, R. N. ( 1981; ). Deteminative methods of light microscopy. In Manual of Methods for General Bacteriology. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  5. Gujer, W. & Zehnder, A. J. B. ( 1983; ). Conversion processes in anaerobic digestion. Water Sci Technol 15, 127–167.
    [Google Scholar]
  6. Harmsen, H. J. M. ( 1996; ). Detection, phylogeny and population dynamics of syntrophic propionate-oxidizing bacteria in anaerobic granular sludge. PhD thesis, Wageningen Agricultural University, Wageningen, The Netherlands.
  7. Harmsen, H. J. M., Van Kuijk, B. L. M., Plugge, C. M., Akkermans, A. D. L., De Vos, W. M. & Stams, A. J. M. ( 1998; ). Syntrophobacter fumaroxidans sp. nov., a syntrophic propionate-degrading sulfate-reducing bacterium. Int J Syst Bacteriol 48, 1383–1387.[CrossRef]
    [Google Scholar]
  8. Hatamoto, M., Imachi, H., Ohashi, A. & Harada, H. ( 2007; ). Identification and cultivation of anaerobic, syntrophic long-chain fatty acid-degrading microbes from mesophilic and thermophilic methanogenic sludges. Appl Environ Microbiol 73, 1332–1340.[CrossRef]
    [Google Scholar]
  9. Hattori, S., Kamagata, Y., Hanada, S. & Shoun, H. ( 2000; ). Thermoacetogenium phaeum gen. nov., sp. nov., a strictly anaerobic, thermophilic, syntrophic acetate-oxidizing bacterium. Int J Syst Evol Microbiol 50, 1601–1609.[CrossRef]
    [Google Scholar]
  10. Imachi, H., Sekiguchi, Y., Kamagata, Y., Ohashi, A. & Harada, H. ( 2000; ). Cultivation and in situ detection of a thermophilic bacterium capable of oxidizing propionate in syntrophic association with hydrogenotrophic methanogens in a thermophilic methanogenic granular sludge. Appl Environ Microbiol 66, 3608–3615.[CrossRef]
    [Google Scholar]
  11. Imachi, H., Sekiguchi, Y., Kamagata, Y., Hanada, S., Ohashi, A. & Harada, H. ( 2002; ). Pelotomaculum thermopropionicum gen. nov., sp. nov., an anaerobic, thermophilic, syntrophic propionate-oxidizing bacterium. Int J Syst Evol Microbiol 52, 1729–1735.[CrossRef]
    [Google Scholar]
  12. Imachi, H., Sekiguchi, Y., Kamagata, Y., Loy, A., Qiu, Y.-L., Hugenholtz, P., Kimura, N., Wagner, M., Ohashi, A. & Harada, H. ( 2006; ). Non-sulfate-reducing, syntrophic bacteria affiliated with Desulfotomaculum cluster I are widely distributed in methanogenic environments. Appl Environ Microbiol 72, 2080–2091.[CrossRef]
    [Google Scholar]
  13. Ishii, S., Kosaka, T., Hori, K., Hotta, Y. & Watanabe, K. ( 2005; ). Coaggregation facilitates interspecies hydrogen transfer between Pelotomaculum thermopropionicum and Methanothermobacter thermautotrophicus. Appl Environ Microbiol 71, 7838–7845.[CrossRef]
    [Google Scholar]
  14. Juteau, P., Côté, V., Duckett, M.-F., Beaudet, R., Lépine, F., Villemur, R. & Bisaillon, J.-G. ( 2005; ). Cryptanaerobacter phenolicus gen. nov., sp. nov., an anaerobe that transforms phenol into benzoate via 4-hydroxybenzoate. Int J Syst Evol Microbiol 55, 245–250.[CrossRef]
    [Google Scholar]
  15. Kaspar, H. P. & Wuhrmann, K. ( 1978; ). Kinetic parameters and relative turnovers of some important catabolic reactions in digesting sludge. Appl Environ Microbiol 36, 1–7.
    [Google Scholar]
  16. Kosaka, T., Uchiyama, T., Ishii, S., Enoki, M., Imachi, H., Kamagata, Y., Ohashi, A., Harada, H., Ikenaga, H. & Watanabe, K. ( 2006; ). Reconstruction and regulation of the central catabolic pathway in the thermophilic propionate-oxidizing syntroph Pelotomaculum thermopropionicum. J Bacteriol 188, 202–210.[CrossRef]
    [Google Scholar]
  17. Liu, Y., Balkwill, D. L., Aldrich, H. C., Drake, G. R. & Boone, D. R. ( 1999; ). Characterization of the anaerobic propionate-degrading syntrophs Smithella propionica gen. nov., sp. nov. and Syntrophobacter wolinii. Int J Syst Bacteriol 49, 545–556.[CrossRef]
    [Google Scholar]
  18. Mah, R. A., Xun, L.-Y., Boone, D. R., Ahring, B., Smith, P. H. & Wilkie, A. ( 1990; ). Methanogenesis from propionate in sludge and enrichment systems. In Microbiology and Biochemistry of Strict Anaerobes Involved in Interspecies Hydrogen Transfer, pp. 99–111. Edited by J.-P. Belaich, M. Bruschi & J.-L. Garcia. New York: Plenum.
  19. Plugge, C. M., Balk, M. & Stams, A. J. M. ( 2002; ). Desulfotomaculum thermobenzoicum subsp. thermosyntrophicum subsp. nov., a thermophilic, syntrophic, propionate-oxidizing, spore-forming bacterium. Int J Syst Evol Microbiol 52, 391–399.
    [Google Scholar]
  20. Qiu, Y.-L., Sekiguchi, Y., Hanada, S., Imachi, H., Tseng, I.-C., Cheng, S.-S., Ohashi, A., Harada, H. & Kamagata, Y. ( 2006; ). Pelotomaculum terephthalaticum sp. nov., and Pelotomaculum isophthalaticum sp. nov.: two anaerobic bacteria that degrade phthalate isomers in syntrophic association with hydrogenotrophic methanogens. Arch Microbiol 185, 172–182.[CrossRef]
    [Google Scholar]
  21. Schink, B. ( 1997; ). Energetics of syntrophic cooperation in methanogenic degradation. Microbiol Mol Biol Rev 61, 262–280.
    [Google Scholar]
  22. Sekiguchi, Y., Kamagata, Y., Syutubo, K., Ohashi, A., Harada, H. & Nakamura, K. ( 1998; ). Phylogenetic diversity of mesophilic and thermophilic granular sludges determined by 16S rRNA gene analysis. Microbiology 144, 2655–2665.[CrossRef]
    [Google Scholar]
  23. Sekiguchi, Y., Kamagata, Y., Nakamura, K., Ohashi, A. & Harada, H. ( 1999; ). Fluorescence in situ hybridization using 16S rRNA-targeted oligonucleotides reveals localization of methanogens and selected uncultured bacteria in mesophilic and thermophilic sludge granules. Appl Environ Microbiol 65, 1280–1288.
    [Google Scholar]
  24. Sekiguchi, Y., Kamagata, Y., Nakamura, K., Ohashi, A. & Harada, H. ( 2000; ). Syntrophothermus lipocalidus gen. nov., sp. nov., a novel thermophilic, syntrophic, fatty-acid-oxidizing anaerobe which utilizes isobutyrate. Int J Syst Evol Microbiol 50, 771–779.[CrossRef]
    [Google Scholar]
  25. Sekiguchi, Y., Imachi, H., Susilorukmi, A., Muramatsu, M., Ohashi, A., Harada, H., Hanada, S. & Kamagata, Y. ( 2006; ). Tepidanaerobacter syntrophicus gen. nov., sp. nov., an anaerobic, moderately themophilic, syntrophic alcohol- and lactate-degrading bacterium isolated from thermophilic digested sludges. Int J Syst Evol Microbiol 56, 1621–1629.[CrossRef]
    [Google Scholar]
  26. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  27. Wallrabenstein, C., Hauschild, E. & Schink, B. ( 1995; ). Syntrophobacter pfennigii sp. nov., new syntrophically propionate-oxidizing anaerobe growing in pure culture with propionate and sulfate. Arch Microbiol 164, 346–352.[CrossRef]
    [Google Scholar]
  28. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64925-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64925-0
Loading

Data & Media loading...

vol. , part 7, pp. 1487-1492

Growth properties of strain MGP on propionate (20 mM) medium. Effect of temperature (a), pH at 37 °C (b) and NaCl concentration (c) on specific growth rate are shown. Error bars indicate standard deviation with triplicate determinations.



IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error