1887

Abstract

A yellow-coloured, aerobic, Gram-negative, rod-shaped bacterial strain, designated B-I, was isolated from the water of a shrimp () mariculture pond in Taiwan. No species with a validly published name showed 16S rRNA gene sequence similarity of more than 96.5 % to this novel isolate. The highest sequence similarities displayed by strain B-I (93.2–96.1 %) were to members of the genus . The phenotypic properties of this organism were consistent with its classification in the genus . The novel isolate could be distinguished from all species by several phenotypic characteristics. The major fatty acids were iso-C (22 %), summed feature 3 (C 7 and/or iso-C 2-OH; 21.3 %), iso-C 3-OH (12.7 %) and iso-C (8.7 %). The G+C content of the genomic DNA was 35.2 mol%. Hence, genotypic and phenotypic data demonstrate that strain B-I should be classified within a novel species in the genus , for which the name sp. nov. is proposed. The type strain is B-I (=BCRC 17590=LMG 23706).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64920-0
2007-05-01
2021-02-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/5/1148.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64920-0&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [CrossRef]
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae , and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  3. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
    [Google Scholar]
  4. Chang S. C., Wang J. T., Vandamme P., Hwang J. H., Chang P. S., Chen W. M. 2004; Chitinimonas taiwanensis gen. nov., sp. nov. a novel chitinolytic bacterium isolated from a freshwater pond for shrimp culture. Syst Appl Microbiol 27:43–49 [CrossRef]
    [Google Scholar]
  5. Chen W. M., Laevens S., Lee T. M., Coenye T., de Vos P., Mergeay M., Vandamme P. 2001; Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int J Syst Evol Microbiol 51:1729–1735 [CrossRef]
    [Google Scholar]
  6. Choi D. H., Kim Y.-G., Hwang C. Y., Yi H., Chun J. 2006; Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 56:635–640 [CrossRef]
    [Google Scholar]
  7. Chung Y. C., Kobayashi T., Kanai H., Akiba T., Kudo T. 1995; Purification and properties of extracellular amylase from the hyperthermophilic archeon Thermococccus profundus DT5432. Appl Environ Microbiol 61:1502–1506
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric DNA-DNA hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  9. Frette L., Jørgensen N. O. G., Irming H., Kroer N. 2004; Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in Skagerrak, Denmark. Int J Syst Evol Microbiol 54:519–524 [CrossRef]
    [Google Scholar]
  10. GCG 1995 Wisconsin Package Version 8.1 Program Manual Madison, WI: Genetics Computer Group;
    [Google Scholar]
  11. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  12. Hansen G. H., Bergh Ø., Michaelsen J., Knappskog D. 1992; Flexibacter ovolyticus sp. nov., a pathogen of eggs and larvae of Atlantic halibut, Hippoglossus hippoglossus L. Int J Syst Bacteriol 42:451–458 [CrossRef]
    [Google Scholar]
  13. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol  3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  14. Jung S.-Y., Oh T.-K., Yoon J.-H. 2006; Tenacibaculum aestuarii sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 56:1577–1581 [CrossRef]
    [Google Scholar]
  15. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  16. Lyman J., Fleming R. H. 1940; Composition of sea water. J Mar Res 3:134–146
    [Google Scholar]
  17. Masuda Y., Tajima K., Ezura Y. 2004; Resuscitation of Tenacibaculum sp., the causative bacterium of spotting disease of sea urchin Strongylocentroutus intermedius , from the viable but non-culturable state. Fish Sci 70:277–284 [CrossRef]
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  19. Powers E. M. 1995; Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61:3756–3758
    [Google Scholar]
  20. Reichenbach H. 1992a; The order Cytophagales . In The Prokaryotes, a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications . , 2nd edn. pp  3631–3675 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
  21. Reichenbach H. 1992b; Flavobacteriaceae fam. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 41. Int J Syst Bacteriol 42:327–329 [CrossRef]
    [Google Scholar]
  22. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  23. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga -like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb.nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp.nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51:1639–1652 [CrossRef]
    [Google Scholar]
  24. Tajima K., Hirano T., Shimizu M., Ezura Y. 1997a; Isolation and pathogenicity of the causative bacterium of spotting disease of sea urchin Strongylocentrotus intermedius . Fish Sci 63:249–252 [CrossRef]
    [Google Scholar]
  25. Tajima K., Hirano T., Nakano K., Ezura Y. 1997b; Taxonomical study of the causative bacterium of spotting disease of sea urchin Strongylocentrotus intermedius . Fish Sci 63:897–900
    [Google Scholar]
  26. Wakabayashi H., Hikida M., Masumura K. 1986; Flexibacter maritimus sp. nov., a pathogen of marine fishes. Int J Syst Bacteriol 36:396–398 [CrossRef]
    [Google Scholar]
  27. Yoon J. H., Kang S. J., Oh T. K. 2005; Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55:793–798 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64920-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64920-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error