1887

Abstract

A phylogenetic tree of the cluster was inferred from a set of concatenated sequences from five housekeeping genes (, , , and ). The relevance of this phylogeny was reinforced by detailed analysis of the congruence of the phylogenies derived from each of the five individual gene sequences. Two subclusters were distinguished. The subcluster comprised subsp. biotypes Small Colony (SC) and Large Colony (LC) and subsp. . The latter two groups could not be clearly separated, which supports previous proposals that they be united into a single taxonomic entity. The subcluster included subsp. , subsp. and sp. bovine group 7 of Leach, a group of strains that remains unassigned. This group constituted a distinct branch within this cluster, supporting its classification as a subspecies of . and clustered in a group that was distinct from and they were all clearly separated from the cluster. In conclusion, this approach has allowed us to assign phylogenetic positions to all members of the cluster and related species and has proved the need to adjust the existing taxonomy. Furthermore, this method may be used as a reference technique to assign an unequivocal position to any particular strain related to this cluster and may lead to the development of new techniques for rapid species identification.

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2007-10-01
2020-11-24
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