1887

Abstract

A phylogenetic tree of the cluster was inferred from a set of concatenated sequences from five housekeeping genes (, , , and ). The relevance of this phylogeny was reinforced by detailed analysis of the congruence of the phylogenies derived from each of the five individual gene sequences. Two subclusters were distinguished. The subcluster comprised subsp. biotypes Small Colony (SC) and Large Colony (LC) and subsp. . The latter two groups could not be clearly separated, which supports previous proposals that they be united into a single taxonomic entity. The subcluster included subsp. , subsp. and sp. bovine group 7 of Leach, a group of strains that remains unassigned. This group constituted a distinct branch within this cluster, supporting its classification as a subspecies of . and clustered in a group that was distinct from and they were all clearly separated from the cluster. In conclusion, this approach has allowed us to assign phylogenetic positions to all members of the cluster and related species and has proved the need to adjust the existing taxonomy. Furthermore, this method may be used as a reference technique to assign an unequivocal position to any particular strain related to this cluster and may lead to the development of new techniques for rapid species identification.

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2007-10-01
2024-12-14
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References

  1. Abu-Groun E. A. M., Taylor R. R., Varsani H., Wadher B. J., Leach R. H., Miles R. J. 1994; Biochemical diversity within the ‘ Mycoplasma mycoides cluster’. Microbiology 140:2033–2042 [CrossRef]
    [Google Scholar]
  2. Askaa G., Ernø H., Ojo M. O. 1978; Bovine mycoplasmas: classification of groups related to Mycoplasma mycoides . Acta Vet Scand 19:166–178
    [Google Scholar]
  3. Atalaia V., Machado M., Franco Frazão F. 1987; Patologia dos pequenos ruminantes infecções em ovinos e caprinos, originadas pelo micoplasma do grupo 7, Leach (Pg. 50). Repos Trab Lab Nac Invest Vet 19:55–60 (in Portuguese
    [Google Scholar]
  4. Christiansen C., Ernø H. 1982; Classification of the F38 group of caprine mycoplasma strains by DNA hybridization. J Gen Microbiol 128:2523–2526
    [Google Scholar]
  5. Christiansen G., Ernø H. 1990; RFLP in rRNA genes of Mycoplasma capricolum , the caprine F38-like group and the bovine serogroup 7. Zentralbl Bakteriol Hyg Suppl 20:479–488
    [Google Scholar]
  6. Clyde W. A. Jr 1983; Growth inhibition tests. In Methods in Mycoplasmology pp 405–410 Edited by Razin S., Tully J. G. New York: Academic Press;
    [Google Scholar]
  7. Cordy D. R., Adler H. E., Yamamoto R. 1955; A pathogenic pleuropneumonialike organism from goats. Cornell Vet 45:50–68
    [Google Scholar]
  8. Costas M., Leach R. H., Mitchelmore D. L. 1987; Numerical analysis of PAGE protein patterns and the taxonomic relationships within the ‘ Mycoplasma mycoides cluster’. J Gen Microbiol 133:3319–3329
    [Google Scholar]
  9. Cottew G. S., Yeats F. R. 1978; Subdivision of Mycoplasma mycoides subsp. mycoides from cattle and goats into two types. Aust Vet J 54:293–296 [CrossRef]
    [Google Scholar]
  10. Cottew G. S., Yeats F. R. 1981; Occurrence of mycoplasmas in clinically normal goats. Aust Vet J 57:52–53 [CrossRef]
    [Google Scholar]
  11. Cottew G. S., Yeats F. R. 1982; Mycoplasmas and mites in the ears of clinically normal goats. Aust Vet J 59:77–81 [CrossRef]
    [Google Scholar]
  12. Cottew G. S., Breard A., DaMassa A. J., Ernø H., Leach R. H., Lefevre P. C., Rodwell A. W., Smith G. R. 1987; Taxonomy of the Mycoplasma mycoides cluster. Isr J Med Sci 23:632–635
    [Google Scholar]
  13. DaMassa A. J., Tully J. G., Rose D. L., Pitcher D., Leach R. H., Cottew G. S. 1994; Mycoplasma auris sp. nov., Mycoplasma cottewii sp. nov. and Mycoplasma yeatsii sp. nov., new sterol-requiring mollicutes from the external ear canals of goats. Int J Syst Bacteriol 44:479–484 [CrossRef]
    [Google Scholar]
  14. Djordjevic S. P., Vilei E. M., Frey J. 2003; Characterization of a chromosomal region of Mycoplasma sp. bovine group 7 strain PG50 encoding a glycerol transport locus ( gtsABC ). Microbiology 149:195–204 [CrossRef]
    [Google Scholar]
  15. Ernø H., Leach R. H., Salih M. M., MacOwan K. J. 1983; The F38-like group, a new group of caprine mycoplasmas?. Acta Vet Scand 24:275–286
    [Google Scholar]
  16. Frey J., Cheng X., Monnerat M. P., Abdo E. M., Krawinkler M., Bölske G., Nicolet J. 1998; Genetic and serological analysis of the immunogenic 67-kDa lipoprotein of Mycoplasma sp. bovine group 7. Res Microbiol 149:55–64 [CrossRef]
    [Google Scholar]
  17. Harasawa R., Hotzel H., Sachse K. 2000; Comparison of the 16S–23S rRNA intergenic spacer regions among strains of the Mycoplasma mycoides cluster, and reassessment of the taxonomic position of Mycoplasma sp. bovine group 7. Int J Syst Evol Microbiol 50:1325–1329 [CrossRef]
    [Google Scholar]
  18. Heldtander M., Pettersson B., Tully J. G., Johansson K.-E. 1998; Sequences of the 16S rRNA genes and phylogeny of the goat mycoplasmas Mycoplasma adleri , Mycoplasma auris , Mycoplasma cottewii and Mycoplasma yeatsii . Int J Syst Bacteriol 48:263–268 [CrossRef]
    [Google Scholar]
  19. Johansson K.-E., Bradbury J. M. 2007; International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Mollicutes. Minutes of the meetings 7 and 12 July 2002. Vienna, Austria. Int J Syst Evol Microbiol 57:181–186 [CrossRef]
    [Google Scholar]
  20. Kibe M. K., Bidwell D. E., Turp P., Smith G. R. 1985; Demonstration of cross-reactive antigens in F38 and related mycoplasmas by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. J Hyg (Lond) 95:95–106 [CrossRef]
    [Google Scholar]
  21. Kim K. S., Ko K. S., Chang M. W., Hahn T. W., Hong S. K., Kook Y. H. 2003; Use of rpoB sequences for phylogenetic study of Mycoplasma species. FEMS Microbiol Lett 226:299–305 [CrossRef]
    [Google Scholar]
  22. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  23. Kusiluka L. J., Ojeniyi B., Friis N. F., Kokotovic B., Ahrens P. 2001; Molecular analysis of field strains of Mycoplasma capricolum subspecies capripneumoniae and Mycoplasma mycoides subspecies mycoides , small colony type isolated from goats in Tanzania. Vet Microbiol 82:27–37 [CrossRef]
    [Google Scholar]
  24. Laws L. 1956; A pleuropneumonia-like organism causing peritonitis in goats. Aust Vet J 32:326–329 [CrossRef]
    [Google Scholar]
  25. Leach R. H. 1967; Comparative studies of mycoplasma of bovine origin. Ann N Y Acad Sci 143:305–316 [CrossRef]
    [Google Scholar]
  26. Leach R. H., Costas M., Mitchelmore D. L. 1989; Relationship between Mycoplasma mycoides subsp. mycoides (‘large-colony’ strains) and M. mycoides subsp. capri , as indicated by numerical analysis of one-dimensional SDS-PAGE protein patterns. J Gen Microbiol 135:2993–3000
    [Google Scholar]
  27. Leach R. H., Ernø H., MacOwan K. J. 1993; Proposal for designation of F38-type caprine mycoplasmas as Mycoplasma capricolum subsp. capripneumoniae subsp. nov. and consequent obligatory relegation of strains currently classified as M. capricolum (Tully, Barile, Edward, Theodore, and Ernø 1974) to an additional new subspecies, M.capricolum subsp. capricolum subsp. nov.. Int J Syst Bacteriol 43: 603–605 [CrossRef]
    [Google Scholar]
  28. Miserez R., Pilloud T., Cheng X., Nicolet J., Griot C., Frey J. 1997; Development of a sensitive nested PCR method for the specific detection of Mycoplasma mycoides subsp. mycoides SC. Mol Cell Probes 11:103–111 [CrossRef]
    [Google Scholar]
  29. Monnerat M. P., Thiaucourt F., Poveda J. B., Nicolet J., Frey J. 1999 Genetic and serological analysis of lipoprotein LppA in Mycoplasma mycoides subsp.mycoides LC and Mycoplasmamycoides subsp. capri . Clin Diagn Lab Immunol 6, 224–230
  30. Olsson B., Bölske G., Bergström K., Johansson K. E. 1990; Analysis of caprine mycoplasmas and mycoplasma infections in goats using two-dimensional electrophoresis and immunoblotting. Electrophoresis 11:861–869 [CrossRef]
    [Google Scholar]
  31. Perrier X., Flori A., Bonnot F. 2003; Data analysis methods. In Genetic Diversity of Cultivated Tropical Plants . pp 43–76 Edited by Hamon P., Seguin M., Perrier X., Glaszmann J. C. Enfield, UK: Science Publishers;
  32. Pettersson B., Leitner T., Ronaghi M., Bölske G., Uhlén M., Johansson K.-E. 1996; Phylogeny of the Mycoplasma mycoides cluster as determined by sequence analysis of the 16S rRNA genes from the two rRNA operons. J Bacteriol 178:4131–4142
    [Google Scholar]
  33. Peyraud A., Woubit S., Poveda J. B., De la Fe C., Mercier P., Thiaucourt F. 2003; A specific PCR for the detection of Mycoplasma putrefaciens , one of the agents of the contagious agalactia syndrome of goats. Mol Cell Probes 17:289–294 [CrossRef]
    [Google Scholar]
  34. Poumarat F., Longchambon D., Martel J. L. 1992; Application of dot immunobinding on membrane filtration (MF dot) to the study of relationships within “ M. mycoides cluster” and within “glucose and arginine-negative cluster” of ruminant mycoplasmas. Vet Microbiol 32:375–390 [CrossRef]
    [Google Scholar]
  35. Rodwell A. W. 1982; Emmy Klieneberger-Nobel Award Lecture: The protein fingerprints of mycoplasmas. Rev Infect Dis 4:Suppl.S8–S17 [CrossRef]
    [Google Scholar]
  36. Rokas A., Williams B. L., King N., Carroll S. B. 2003; Genome-scale approaches to resolving incongruence in molecular phylogenies. Nature 425:798–804 [CrossRef]
    [Google Scholar]
  37. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  38. Salih M. M., Ernø H., Simonsen V. 1983; Electrophoretic analysis of isoenzymes of mycoplasma species. Acta Vet Scand 24:14–33
    [Google Scholar]
  39. Santos S. R., Ochman H. 2004; Identification and phylogenetic sorting of bacterial lineages with universally conserved genes and proteins. Environ Microbiol 6:754–759 [CrossRef]
    [Google Scholar]
  40. Schwan T. G., Raffel S. J., Schrumpf M. E., Policastro P. F., Rawlings J. A., Lane R. S., Breitschwerdt E. B., Porcella S. F. 2005; Phylogenetic analysis of the spirochetes Borrelia parkeri and Borrelia turicatae and the potential for tick-borne relapsing fever in Florida. J Clin Microbiol 43:3851–3859 [CrossRef]
    [Google Scholar]
  41. Simmons G. C., Johnston L. A. Y. 1963; Arthritis in calves caused by Mycoplasma species. Aust Vet J 39:11–14 [CrossRef]
    [Google Scholar]
  42. Taylor T. K., Bashiruddin J. B., Gould A. R. 1992; Relationships between members of the Mycoplasma mycoides cluster as shown by DNA probes and sequence analysis. Int J Syst Bacteriol 42:593–601 [CrossRef]
    [Google Scholar]
  43. Thiaucourt F., Bölske G. 1996; Contagious caprine pleuropneumonia and other pulmonary mycoplasmoses of sheep and goats. Rev Sci Tech 15:1397–1414
    [Google Scholar]
  44. Thiaucourt F., Lorenzon S., David A., Breard A. 2000; Phylogeny of the Mycoplasma mycoides cluster as shown by sequencing of a putative membrane protein gene. Vet Microbiol 72:251–268 [CrossRef]
    [Google Scholar]
  45. Thigpen J. E., Cottew G. S., Yeats F., McGhee C. E., Rose D. L. 1983; Growth characteristics of large- and small-colony types of Mycoplasma mycoides subsp. mycoides on 5 % sheep blood agar. J Clin Microbiol 18:956–960
    [Google Scholar]
  46. Tully J. G., Barile M. F., Edward D. G. ff., Theodore T. S., Ernø H. 1974; Characterization of some caprine mycoplasmas, with proposals for new species, Mycoplasma capricolum and Mycoplasma putrefaciens . J Gen Microbiol 85:102–120 [CrossRef]
    [Google Scholar]
  47. Ushida C., Yoshida A., Miyakawa Y., Ara Y., Muto A. 2003; Distribution of the MCS4 RNA genes in mycoplasmas belonging to the Mycoplasma mycoides cluster. Gene 314:149–155 [CrossRef]
    [Google Scholar]
  48. Vilei E. M., Korczak B. M., Frey J. 2006; Mycoplasma mycoides subsp. capri and Mycoplasma mycoides subsp. mycoides LC can be grouped into a single subspecies. Vet Res 37:779–790 [CrossRef]
    [Google Scholar]
  49. Weisburg W. G., Tully J. G., Rose D. L., Petzel J. P., Oyaizu H., Yang D., Mandelco L., Sechrest J., Lawrence T. G. other authors 1989; A phylogenetic analysis of the mycoplasmas: basis for their classification. J Bacteriol 171:6455–6467
    [Google Scholar]
  50. Woese C. R. 1987; Bacterial evolution. Microbiol Rev 51:221–271
    [Google Scholar]
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