1887

Abstract

Four Gram-positive, catalase-positive, short rod- or coccoid-shaped bacterial strains, R27, R44, R45 and R47, were isolated from an enrichment culture with diesel oil-degradation activity and their taxonomic positions were investigated using a polyphasic approach. Phenotypic, phylogenetic and genetic similarities indicated that strains R27, R44, R45 and R47 belong to the same species. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four strains form a distinct evolutionary lineage within the family . The novel four strains had cell-wall peptidoglycan based on -diaminopimelic acid, MK-9(H) as the predominant menaquinone and anteiso-C as the major cellular fatty acid. The DNA G+C contents were 68.8–69.2 mol%. These chemotaxonomic properties, together with phylogenetic distinctiveness, distinguish the four novel strains from recognized members of the family . On the basis of phenotypic, chemotaxonomic, phylogenetic and genetic data, strains R27, R44, R45 and R47 are classified as representatives of a new genus and novel species, gen. nov., sp. nov., within the family . The type strain of sp. nov. is R27 (=KCTC 19182=JCM 14204).

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2007-09-01
2020-12-05
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