1887

Abstract

Two Gram-negative, non-spore-forming, motile and helical-shaped bacterial strains, K92 and K93, were isolated from sludge from a dye works in Korea, and their taxonomic positions were investigated by means of a polyphasic approach. Strains K92 and K93 grew optimally at 37 °C and pH 7.0–8.0 in the presence of 0.5 % (w/v) NaCl. They contained Q-10 as the predominant ubiquinone and C 7 as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and two unidentified amino-group-containing lipids that were ninhydrin-positive. Their DNA G+C contents were 70.0 mol%. The 16S rRNA gene sequences of K92 and K93 showed no differences, and the two strains had a mean DNA–DNA relatedness of 93 %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains K92 and K93 formed a distinct evolutionary lineage within the . The 16S rRNA gene sequences of strains K92 and K93 exhibited similarity values of less than 91.5 % with respect to the 16S rRNA gene sequences of other members of the . The two strains were distinguishable from phylogenetically related genera through differences in several phenotypic properties. On the basis of the phenotypic, phylogenetic and genetic data, strains K92 and K93 represent a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is K92 (=KCTC 12839=JCM 14346).

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2007-06-01
2019-12-11
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Transmission electron micrograph of a cell of strain K92 from an exponentially growing culture. Bar, 1 µm.

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Cellular fatty acid compositions of strains K92 and K93 and strains of and . [PDF]

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