1887

Abstract

A Gram-negative, non-spore-forming, rod-shaped -like bacterial strain, K110, was isolated from wastewater collected from a dye works in Korea and was subjected to a polyphasic taxonomic analysis. Strain K110 grew optimally at pH 7.0–-8.0 and 37 °C in the presence of 0.5 % (w/v) NaCl. It contained Q-8 as the predominant ubiquinone and C, cyclo C and cyclo C 8 as the major fatty acids. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids. The DNA G+C content was 67.4 mol%. In a neighbour-joining phylogenetic tree constructed on the basis of 16S rRNA gene sequences, strain K110 joined , the sole species of the genus, at a bootstrap confidence level of 100 %. Strain K110 exhibited a 16S rRNA gene sequence similarity of 99.4 % with respect to the type strain of . Although strain K110 was found to be similar to in terms of phenotypic properties, it differed in terms of motility, polar lipids, DNA–DNA relatedness and repetitive extragenic palindromic PCR genomic fingerprinting patterns. On the basis of phenotypic, phylogenetic and genetic data, strain K110 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is K110 (=KCTC 12838=JCM 14330).

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2007-06-01
2019-10-15
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vol. , part 6, pp. 1188 - 1191

Cellular fatty acid compositions (%) of strain K110 and DSM 13608 .

rep-PCR fingerprints generated by REP, BOX and (GTG) PCR primers showing relationships between sp. nov. K110 and DSM 13608 .

[PDF file of Supplementary Table S1 and Supplementary Fig. S1](72 KB)



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