A Gram-negative, pleomorphic, extremely halophilic archaeon, designated strain XH-70T, was isolated from the saline Lake Xilinhot, in Inner Mongolia, China. It formed small (0.9–1.5 mm), red-pigmented, elevated colonies on agar medium. The strain required at least 2.5 M NaCl and 5 mM Mg2+ for growth. The 16S rRNA gene sequence analysis indicated that strain XH-70T belongs to the family Halobacteriaceae, showing 99.5 % similarity to the type strain of Halovivax asiaticus and 94.7 and 94.6 % similarity, respectively, to the type strains of Natronococcus amylolyticus and Natronococcus occultus. Polar lipid analysis supported the placement of strain XH-70T in the genus Halovivax. DNA–DNA hybridization studies (32 % with Halovivax asiaticus CGMCC 1.4248T), as well as biochemical and physiological characterization, allowed strain XH-70T to be differentiated from Halovivax asiaticus. A novel species, Halovivax ruber sp. nov., is therefore proposed to accommodate this strain. The type strain is XH-70T (=CGMCC 1.6204T=DSM 18193T=JCM 13892T).
ArahalD. R.,
DewhirstF. E.,
PasterB. J.,
VolcaniB. E.,
VentosaA.1996; Phylogenetic analyses of some extremely halophilic archaea isolated from Dead Sea water, determined on the basis of their 16S rRNA sequences. Appl Environ Microbiol 62:3779–3786
ArahalD. R.,
GarcíaM. T.,
LudwigW.,
SchleiferK. H.,
VentosaA.2001; Transfer of Halomonas canadensis and Halomonas israelensis to the genus Chromohalobacter as Chromohalobacter canadensis comb. nov. and Chromohalobacter israelensis comb. nov. Int J Syst Evol Microbiol 51:1443–1448
CastilloA. M.,
GutiérrezM. C.,
KamekuraM.,
MaY.,
CowanD. A.,
JonesB. E.,
GrantW. D.,
VentosaA.2006Halovivax asiaticus gen. nov., sp. nov., a novel extremely halophilic archaeon isolated from Inner Mongolia, China. Int J Syst Evol Microbiol 56, 765–770 [CrossRef]
De LeyJ.,
TijtgatR.1970; Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek 36:461–474[CrossRef]
GrantW. D.,
KamekuraM.,
McGenityT. J.,
VentosaA.2001; Order I. Halobacteriales Grant and Larsen 1989b, 495VP (Effective publication: Grant and Larsen 1989a, 2216). In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp 294–334 Edited by
BooneD. R.,
CastenholzR. W.,
GarrityG. M.
New York: Springer;
JohnsonJ. L.1994; Similarity analysis of DNAs. In Methods for General and Molecular Biotechnology
. pp 655–681 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
KamekuraM.1993; Lipids of extreme halophiles. In The Biology of Halophilic Bacteria pp 135–161 Edited by
VreelandR. H.,
HochsteinL. I.
Boca Raton, FL: CRC Press;
Lopez-GarciaP.,
MoreiraD.,
Lopez-LopezA.,
Rodríguez-ValeraF.2001; A novel haloarchaeal-related lineage is widely distributed in deep oceanic regions. Environ Microbiol 3:72–78[CrossRef]
MarmurJ.,
DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118[CrossRef]
OrenA.,
VentosaA.,
GrantW. D.1997; Proposed minimal standards for description of new taxa in the order Halobacteriales
. Int J Syst Bacteriol 47:233–238[CrossRef]
OwenR. J.,
HillL. R.1979; The estimation of base compositions, base pairing and genome size of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists , 2nd edn. pp 217–296 Edited by
SkinnerF. A.,
LovelockD. W.
London: Academic Press;
VentosaA.2006; Unusual micro-organisms from unusual habitats: hypersaline environments. In Prokaryotic Diversity: Mechanisms and Significance (Society for General Microbiology Symposium, no. 66) pp 223–253 Edited by
LoganN. A.,
Lappin-ScottH. M.,
OystonP. C. F.
Cambridge: Cambridge University Press;
VentosaA.,
GutiérrezM. C.,
KamekuraM.,
ZvyagintsevaI. S.,
OrenA.2004; Taxonomic study of Halorubrum distributum and proposal of Halorubrum terrestre sp. nov. Int J Syst Evol Microbiol 54:389–392[CrossRef]
WilsonK.1987; Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology pp. 2.4.1–2.4.2 Edited by
AusubelF. M.,
BentR.,
KingstonR. E.,
MooreD. D.,
SeidmanJ. G.,
SmithJ. A,
StruhlK.
New York: Wiley;