1887

Abstract

Two Gram-negative, motile, non-spore-forming bacterial strains, SW-353 and SW-369, were isolated from seawater from the East Sea, Korea, and their taxonomic positions were investigated by means of a polyphasic study. Strains SW-353 and SW-369 grew optimally at 30–37 °C and pH 7.0–8.0. Strains SW-353 and SW-369 contained Q-8 as the predominant ubiquinone and contained C, C 7 and C 7 and/or iso-C 2-OH as the major fatty acids. The DNA G+C contents were 50.1 and 50.5 mol%. Strains SW-353 and SW-369 exhibited no differences in their 16S rRNA gene sequences and showed a mean DNA–DNA relatedness level of 91 %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains SW-353 and SW-369 belong to the genus . Similarity values between the 16S rRNA gene sequences of the two isolates and the type strains of the recognized species were in the range 96.6–97.9 %. DNA–DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strains SW-353 and SW-369 differ from the recognized species. On the basis of phenotypic, phylogenetic and genetic data, therefore, strains SW-353 and SW-369 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SW-353 (=KCTC 12840=JCM 14331).

Erratum

An erratum has been published for this content:
sp. nov., isolated from seawater of the East Sea in Korea
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64898-0
2007-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/7/1386.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64898-0&mimeType=html&fmt=ahah

References

  1. Baumann P., Baumann L. 1981 The marine Gram-negative eubacteria: genera Photobacterium ,Beneckea , Alteromonas , Pseudomonas , and Alcaligenes . In The Prokaryotes pp  1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
  2. Brettar I., Christen R., Höfle M. G. 2002; Rheinheimera baltica gen. nov., sp. nov., a blue-coloured bacterium isolated from the central Baltic Sea. Int J Syst Evol Microbiol 52:1851–1857 [CrossRef]
    [Google Scholar]
  3. Brettar I., Christen R., Höfle M. G. 2006; Rheinheimera perlucida sp. nov., a marine bacterium of the Gammaproteobacteria isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 56:2177–2183 [CrossRef]
    [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. 2001 Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 511997–2006 [CrossRef]
  5. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49:25–68 [CrossRef]
    [Google Scholar]
  6. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  8. Komagata K., Suzuki K. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–203
    [Google Scholar]
  9. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  10. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  11. Romanenko L. A., Uchino M., Falsen E., Zhukova N. V., Mikhailov V. V., Uchimura T. 2003; Rheinheimera pacifica sp. nov., a novel halotolerant bacterium isolated from deep sea water of the Pacific. Int J Syst Evol Microbiol 53:1973–1977 [CrossRef]
    [Google Scholar]
  12. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  13. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  14. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium : new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942
    [Google Scholar]
  15. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  16. Vogel B. F., Venkateswaran K., Christensen H., Falsen E., Christiansen G., Gram L. 2000; Polyphasic taxonomic approach in the description of Alishewanella fetalis gen. nov., sp. nov., isolated from a human foetus. Int J Syst Evol Microbiol 501133–1142 [CrossRef]
    [Google Scholar]
  17. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  18. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  19. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  20. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64898-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64898-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error