1887

Abstract

L. is a pasture legume species that forms a highly specific nitrogen-fixing symbiotic interaction with a group of bacteria that belong to . These mesorhizobia have >98.8 % sequence similarity to and for the 16S rRNA gene (1440 bp) and >99.3 % sequence similarity to for the gene (300 bp), and strain WSM1271 has 100 % sequence similarity to for (600 bp). Strain WSM1271 had 85 % relatedness to LMG 14989 and 50 % relatedness to LMG 6125 when DNA–DNA hybridization was performed. WSM1271 also had a similar cellular fatty acid profile to . These results are strong evidence that the mesorhizobia and belong to the same group of bacteria. Significant differences were revealed between the mesorhizobia and in growth conditions, antibiotic resistance and carbon source utilization. The G+C content of the DNA of WSM1271 was 62.7 mol%, compared to 63–64 mol% for . The mesorhizobia contained a plasmid (∼500 bp), but the symbiotic genes were detected on a mobile symbiosis island and considerable variation was present in the symbiotic genes of mesorhizobia and . There was <78.6 % sequence similarity for and <66.9 % for between mesorhizobia and . Moreover, the mesorhizobia did not nodulate the legume host of , , and did not nodulate . These significant differences observed between mesorhizobia and warrant the proposal of a novel biovar for mesorhizobia within . The name biovar biserrulae is proposed, with strain WSM1271 (=LMG 23838=HAMBI 2942) as the reference strain.

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2007-05-01
2019-10-15
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