1887

Abstract

A Gram-positive, yellow-pigmented, non-motile and rod-shaped or coccoid bacterial strain, DS-61, was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic approach. The strain grew optimally at pH 6.5–7.5 and 25 °C in the presence of 1.0 % (w/v) NaCl. Strain DS-61 had peptidoglycan of the type based on -Lys–-Thr–-Asp and contained galactose as the only whole-cell sugar. MK-9(H) was the predominant menaquinone and anteiso-C and iso-C were the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid. The DNA G+C content was 72.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DS-61 is most closely affiliated to the genus , clustering with and . The levels of 16S rRNA gene sequence similarity between strain DS-61 and the type strains of and were 97.4–97.6 %. DNA–DNA relatedness data and differential phenotypic properties demonstrated that strain DS-61 is distinguishable from these two recognized species. On the basis of phenotypic, phylogenetic and genetic data, strain DS-61 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DS-61 (=KCTC 19206=DSM 18665). An emended description of the genus is given.

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2007-11-01
2021-10-24
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