1887

Abstract

A strain isolated from pleural fluid of a patient with suppurative pleuritis (strain GTC 3021) was characterized in terms of its phenotypic and biochemical features, cellular fatty acid profile and phylogenetic position based on 16S rRNA gene sequence analysis. 16S rRNA gene sequence analysis showed that the isolate was a member of the genus . The isolate was related to ATCC 51261 with about 92 % 16S rRNA gene sequence similarity. The strain was an obligately anaerobic, non-pigmenting, non-spore-forming, non-motile, Gram-negative rod. Although the phenotypic and biochemical characteristics of the strain were similar to those of JCM 12259, the cellular fatty acid composition of the isolate was significantly different from that of JCM 12259 (C 9 and anteiso-C fatty acid content). Based on these data, we propose a novel species, sp. nov., with the type strain GTC 3021 (=JCM 14110 =CCUG 54350). The G+C content of the type strain is 45.4 mol%.

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2007-08-01
2019-09-15
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References

  1. Downes, J., Sutcliffe, I. C., Hofstad, T. & Wade, W. G. ( 2006; ). Prevotella bergensis sp. nov., isolated from human infections. Int J Syst Evol Microbiol 56, 609–612.[CrossRef]
    [Google Scholar]
  2. Felsenstein, J. ( 1985; ). Confidence limits of phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  3. Holdeman, L. V., Cato, E. P. & Moore, W. E. C. ( 1977; ). Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  4. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  5. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  6. Kuykendall, L. D., Roy, M. A., O'Neill, J. J. & Devine, T. E. ( 1988; ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 38, 358–361.[CrossRef]
    [Google Scholar]
  7. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  8. Miller, L. T. ( 1982; ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16, 584–586.
    [Google Scholar]
  9. Moore, L. V. H., Johnson, J. L. & Moore, W. E. C. ( 1994; ). Descriptions of Prevotella tannerae sp. nov. and Prevotella enoeca sp. nov. from the human gingival crevice and emendation of the description of Prevotella zoogleoformans. Int J Syst Bacteriol 44, 599–602.[CrossRef]
    [Google Scholar]
  10. Saito, H. & Miura, K. ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef]
    [Google Scholar]
  11. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  12. Sakamoto, M., Suzuki, M., Umeda, M., Ishikawa, I. & Benno, Y. ( 2002; ). Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov. Int J Syst Evol Microbiol 52, 841–849.[CrossRef]
    [Google Scholar]
  13. Sakamoto, M., Suzuki, M., Huang, Y., Umeda, M., Ishikawa, I. & Benno, Y. ( 2004; ). Prevotella shahii sp. nov. and Prevotella salivae sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol 54, 877–883.[CrossRef]
    [Google Scholar]
  14. Sakamoto, M., Huang, Y., Umeda, M., Ishikawa, I. & Benno, Y. ( 2005a; ). Prevotella multiformis sp. nov., isolated from human subgingival plaque. Int J Syst Evol Microbiol 55, 815–819.[CrossRef]
    [Google Scholar]
  15. Sakamoto, M., Umeda, M., Ishikawa, I. & Benno, Y. ( 2005b; ). Prevotella multisaccharivorax sp. nov., isolated from human subgingival plaque. Int J Syst Evol Microbiol 55, 1839–1843.[CrossRef]
    [Google Scholar]
  16. Shah, H. N. ( 1992; ). The genus Bacteroides and related taxa. In The Prokaryotes, 2nd edn, pp. 3593–3607. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  17. Shah, H. N. & Collins, M. D. ( 1990; ). Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides. Int J Syst Bacteriol 40, 205–208.[CrossRef]
    [Google Scholar]
  18. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  19. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  20. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
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