1887

Abstract

This paper reports on a isolate of bovine origin and its identification as belonging to a previously unknown serovar, for which the name Topaz is proposed. The isolate (94-79970/3) was cultured from bovine urine from a north Queensland dairy farm in Australia. Strain 94-79970/3 grew at 30 °C in Ellinghausen McCullough Johnson Harris (EMJH) medium but failed to grow at 13 °C in EMJH medium or in the presence of 8-azaguanine. Serologically, strain 94-79970/3 produced titres against the serovar Tarassovi, the reference strain for the Tarassovi serogroup; however, no significant titres to any other serovars within the serogroup were obtained. Using 16S rRNA and DNA gyrase subunit B gene analysis, strain 94-79970/3 was identified as a member of the species . We propose that the serovar be named Topaz, after the location where the original isolate was obtained. The reference strain for this serovar is 94-79970/3 (=KIT 94-79970/3=LT722).

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2008-10-01
2024-04-16
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References

  1. Bharti, A. R., Nally, J. E., Ricaldi, J. N., Matthias, M. A., Diaz, M. M., Lovett, M. A., Levett, P. N., Gilman, R. H., Willig, M. R. & other authors(2003). Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis 3, 757–771.[CrossRef] [Google Scholar]
  2. Brenner, D. J., Kaufmann, A. F., Sulzer, K. R., Steigerwalt, A. G., Rogers, F. C. & Weyant, R. S.(1999). Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies. Int J Syst Bacteriol 49, 839–858.[CrossRef] [Google Scholar]
  3. Faine, S., Adler, B. & Bolin, C.(1999). Leptospira and leptospirosis. Melbourne, Australia: MediSci.
  4. Graves, S. & Faine, S.(1970). Antileptospiral agglutinins produced in rabbits. Bull World Health Organ 43, 579–587. [Google Scholar]
  5. Johnson, R. C. & Rogers, P.(1964). Differentiation of pathogenic and saprophytic Leptospires with 8-azaguanine. J Bacteriol 88, 1618–1623. [Google Scholar]
  6. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  7. Kmety, E. & Dikken, H.(1993). Classification of the species Leptospira interrogans and history of its serovars, 1st edn. Groningen, Netherlands: University Press Groningen.
  8. Kmety, E., Galton, M. M. & Sulzer, C. R.(1970). Further standardization of the agglutinin-absorption test in the serology of leptospires. Bull World Health Organ 42, 733–738. [Google Scholar]
  9. Levett, P. N.(2001). Leptospirosis. Clin Microbiol Rev 14, 296–326.[CrossRef] [Google Scholar]
  10. Levett, P. N. & Smythe, L.(2006). International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Leptospiraceae. Minutes of the closed meeting, 12 and 13 November 2005, Chiang Mai, Thailand. Int J Syst Evol Microbiol 56, 2019–2020.[CrossRef] [Google Scholar]
  11. Levett, P. N., Morey, R. E., Galloway, R. L. & Steigerwalt, A. G.(2006).Leptospira broomii sp. nov., isolated from humans with leptospirosis. Int J Syst Evol Microbiol 56, 671–673.[CrossRef] [Google Scholar]
  12. McClintock, C. S., McGowan, M. R., Corney, B. G., Colley, J., Smythe, L., Dohnt, M. & Woodrow, M.(1993). Isolation of Leptospira interrogans serovars hardjo and zanoni from a dairy herd in north Queensland. Aust Vet J 70, 393–394.[CrossRef] [Google Scholar]
  13. Perolat, P., Chappel, R. J., Adler, B., Baranton, G., Bulach, D. M., Billinghurst, M. L., Letocart, M., Merien, F. & Serrano, M. S.(1998).Leptospira fainei sp. nov., isolated from pigs in Australia. Int J Syst Bacteriol 48, 851–858.[CrossRef] [Google Scholar]
  14. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  15. Slack, A. T., Symonds, M. L., Dohnt, M. F. & Smythe, L. D.(2006a). The epidemiology of leptospirosis and the emergence of Leptospira borgpetersenii serovar Arborea in Queensland, Australia, 1998–2004. Epidemiol Infect 134, 1217–1225.[CrossRef] [Google Scholar]
  16. Slack, A. T., Symonds, M. L., Dohnt, M. F. & Smythe, L. D.(2006b). Identification of pathogenic Leptospira species by conventional or real-time PCR and sequencing of the DNA gyrase subunit B encoding gene. BMC Microbiol 6, 95[CrossRef] [Google Scholar]
  17. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  18. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  19. Yasuda, P. H., Steigerwalt, A. G., Sulzer, K. R., Kaufmann, A. F., Rogers, F. & Brenner, D. J.(1987). Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species. Int J Syst Bacteriol 37, 407–415.[CrossRef] [Google Scholar]
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