This paper reports on a isolate of bovine origin and its identification as belonging to a previously unknown serovar, for which the name Topaz is proposed. The isolate (94-79970/3) was cultured from bovine urine from a north Queensland dairy farm in Australia. Strain 94-79970/3 grew at 30 °C in Ellinghausen McCullough Johnson Harris (EMJH) medium but failed to grow at 13 °C in EMJH medium or in the presence of 8-azaguanine. Serologically, strain 94-79970/3 produced titres against the serovar Tarassovi, the reference strain for the Tarassovi serogroup; however, no significant titres to any other serovars within the serogroup were obtained. Using 16S rRNA and DNA gyrase subunit B gene analysis, strain 94-79970/3 was identified as a member of the species . We propose that the serovar be named Topaz, after the location where the original isolate was obtained. The reference strain for this serovar is 94-79970/3 (=KIT 94-79970/3=LT722).


Article metrics loading...

Loading full text...

Full text loading...



  1. Bharti, A. R., Nally, J. E., Ricaldi, J. N., Matthias, M. A., Diaz, M. M., Lovett, M. A., Levett, P. N., Gilman, R. H., Willig, M. R. & other authors(2003). Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis 3, 757–771.[CrossRef] [Google Scholar]
  2. Brenner, D. J., Kaufmann, A. F., Sulzer, K. R., Steigerwalt, A. G., Rogers, F. C. & Weyant, R. S.(1999). Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies. Int J Syst Bacteriol 49, 839–858.[CrossRef] [Google Scholar]
  3. Faine, S., Adler, B. & Bolin, C.(1999). Leptospira and leptospirosis. Melbourne, Australia: MediSci.
  4. Graves, S. & Faine, S.(1970). Antileptospiral agglutinins produced in rabbits. Bull World Health Organ 43, 579–587. [Google Scholar]
  5. Johnson, R. C. & Rogers, P.(1964). Differentiation of pathogenic and saprophytic Leptospires with 8-azaguanine. J Bacteriol 88, 1618–1623. [Google Scholar]
  6. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  7. Kmety, E. & Dikken, H.(1993). Classification of the species Leptospira interrogans and history of its serovars, 1st edn. Groningen, Netherlands: University Press Groningen.
  8. Kmety, E., Galton, M. M. & Sulzer, C. R.(1970). Further standardization of the agglutinin-absorption test in the serology of leptospires. Bull World Health Organ 42, 733–738. [Google Scholar]
  9. Levett, P. N.(2001). Leptospirosis. Clin Microbiol Rev 14, 296–326.[CrossRef] [Google Scholar]
  10. Levett, P. N. & Smythe, L.(2006). International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Leptospiraceae. Minutes of the closed meeting, 12 and 13 November 2005, Chiang Mai, Thailand. Int J Syst Evol Microbiol 56, 2019–2020.[CrossRef] [Google Scholar]
  11. Levett, P. N., Morey, R. E., Galloway, R. L. & Steigerwalt, A. G.(2006).Leptospira broomii sp. nov., isolated from humans with leptospirosis. Int J Syst Evol Microbiol 56, 671–673.[CrossRef] [Google Scholar]
  12. McClintock, C. S., McGowan, M. R., Corney, B. G., Colley, J., Smythe, L., Dohnt, M. & Woodrow, M.(1993). Isolation of Leptospira interrogans serovars hardjo and zanoni from a dairy herd in north Queensland. Aust Vet J 70, 393–394.[CrossRef] [Google Scholar]
  13. Perolat, P., Chappel, R. J., Adler, B., Baranton, G., Bulach, D. M., Billinghurst, M. L., Letocart, M., Merien, F. & Serrano, M. S.(1998).Leptospira fainei sp. nov., isolated from pigs in Australia. Int J Syst Bacteriol 48, 851–858.[CrossRef] [Google Scholar]
  14. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  15. Slack, A. T., Symonds, M. L., Dohnt, M. F. & Smythe, L. D.(2006a). The epidemiology of leptospirosis and the emergence of Leptospira borgpetersenii serovar Arborea in Queensland, Australia, 1998–2004. Epidemiol Infect 134, 1217–1225.[CrossRef] [Google Scholar]
  16. Slack, A. T., Symonds, M. L., Dohnt, M. F. & Smythe, L. D.(2006b). Identification of pathogenic Leptospira species by conventional or real-time PCR and sequencing of the DNA gyrase subunit B encoding gene. BMC Microbiol 6, 95[CrossRef] [Google Scholar]
  17. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  18. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  19. Yasuda, P. H., Steigerwalt, A. G., Sulzer, K. R., Kaufmann, A. F., Rogers, F. & Brenner, D. J.(1987). Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species. Int J Syst Bacteriol 37, 407–415.[CrossRef] [Google Scholar]

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error