1887

Abstract

Eleven strains of a hitherto unknown, Gram-negative, anaerobic coccus were recovered from various human clinical samples of patients hospitalized in two geographically distant French hospitals. These strains displayed the morphology and growth characteristics of those related to the genus . The clinical isolates shared at least 99.9 and 99.7 % of their nucleotide positions in the 16S and 23S rRNA gene sequences, respectively. They displayed 95.6 and 88.9 % 16S and 23S rRNA gene sequence similarities, respectively, with . The 16S rRNA-based phylogeny revealed that all the clinical isolates grouped in a statistically well supported cluster separate from . Enzymic activity profiles as well as metabolic end product patterns, including propionic acid production, differentiated the novel bacteria from . Finally, phenotypic, genotypic and phylogenetic data, including large-scale chromosome structure and DNA G+C content, supported the proposal of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ADV 255.99 (=AIP 283.01=CIP 108586=CCUG 50930).

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2007-10-01
2019-10-16
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References

  1. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Anthony, R. M., Brown, T. J. & French, G. L. ( 2000; ). Rapid diagnosis of bacteremia by universal amplification of 23S ribosomal DNA followed by hybridization to an oligonucleotide array. J Clin Microbiol 38, 781–788.
    [Google Scholar]
  3. Both, B., Buckel, W., Kroppenstedt, R. & Stackebrandt, E. ( 1992; ). Phylogenetic and chemotaxonomic characterization of Acidaminococcus fermentans. FEMS Microbiol Lett 76, 7–11.
    [Google Scholar]
  4. Carlier, J.-P. ( 1985; ). Gas chromatography of fermentation products: its application in diagnosis of anaerobic bacteria. Bull Inst Pasteur 83, 57–69.
    [Google Scholar]
  5. Carlier, J.-P., Marchandin, H., Jumas-Bilak, E., Lorin, V., Henry, C., Carrière, C. & Jean-Pierre, H. ( 2002; ). Anaeroglobus geminatus gen. nov., sp. nov., a novel member of the family Veillonellaceae. Int J Syst Evol Microbiol 52, 983–986.[CrossRef]
    [Google Scholar]
  6. Chatterjee, B. D. & Chakraborti, C. K. ( 1995; ). Non-sporing anaerobes in certain surgical group of patients. J Indian Med Assoc 93, 333–339.
    [Google Scholar]
  7. Cook, G. M., Rainey, F. A., Chen, G., Stackebrandt, E. & Russell, J. B. ( 1994; ). Emendation of the description of Acidaminococcus fermentans, a trans-aconitate- and citrate-oxidizing bacterium. Int J Syst Bacteriol 44, 576–578.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Fuller, R. ( 1966; ). Some morphological and physiological characteristics of gram-negative anaerobic bacteria isolated from the alimentary tract of the pig. J Appl Bacteriol 29, 375–379.[CrossRef]
    [Google Scholar]
  10. Galán, J. C., Reig, M., Navas, A., Baquero, F. & Blázquez, J. ( 2000; ). ACI-1 from Acidaminococcus fermentans: characterization of the first β-lactamase in anaerobic cocci. Antimicrob Agents Chemother 44, 3144–3149.[CrossRef]
    [Google Scholar]
  11. Garrity, G. M. & Holt, J. G. ( 2001; ). Taxonomic outline of the Archaea and Bacteria. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 155–166. Edited by D. R. Boone & R. W. Castenholz. New York: Springer.
  12. Goldstein, E. J., Citron, D. M., Vreni Merriam, C., Warren, Y. & Tyrrell, K. L. ( 2000; ). Comparative in vitro activities of ertapenem (MK-0826) against 1,001 anaerobes isolated from human intra-abdominal infections. Antimicrob Agents Chemother 44, 2389–2394.[CrossRef]
    [Google Scholar]
  13. Holdeman, L. V., Cato, E. P. & Moore, W. E. C. ( 1977; ). Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  14. Jousimies-Somer, H. R., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. & Finegold, S. M. ( 2002; ). Wadworth-KTL Anaerobic Bacteriology Manual, 6th edn. Belmont, CA: Star Publishing Company.
  15. Jumas-Bilak, E., Jean-Pierre, H., Carlier, J.-P., Teyssier, C., Bernard, K., Gay, B., Campos, J., Morio, F. & Marchandin, H. ( 2005; ). Dialister micraerophilus sp. nov. and Dialister propionicifaciens sp. nov., isolated from human clinical samples. Int J Syst Evol Microbiol 55, 2471–2478.[CrossRef]
    [Google Scholar]
  16. Kishino, H. & Hasegawa, M. ( 1989; ). Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in Hominoidea. J Mol Evol 29, 170–179.[CrossRef]
    [Google Scholar]
  17. Kluge, A. & Farris, J. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  18. Liu, S.-L., Schryvers, A. B., Sanderson, K. E. & Johnston, R. N. ( 1999; ). Bacterial phylogenetic clusters revealed by genome structure. J Bacteriol 181, 6747–6755.
    [Google Scholar]
  19. Marchandin, H., Jean-Pierre, H., Carrière, C., Canovas, F., Darbas, H. & Jumas-Bilak, E. ( 2001; ). Prosthetic joint infection due to Veillonella dispar. Eur J Clin Microbiol Infect Dis 20, 340–342.
    [Google Scholar]
  20. Marchandin, H., Jumas-Bilak, E., Gay, B., Teyssier, C., Jean-Pierre, H., Siméon de Buochberg, M., Carrière, C. & Carlier, J.-P. ( 2003a; ). Phylogenetic analysis of some Sporomusa sub-branch members isolated from human clinical specimens: description of Megasphaera micronuciformis sp nov. Int J Syst Evol Microbiol 53, 547–553.[CrossRef]
    [Google Scholar]
  21. Marchandin, H., Teyssier, C., Siméon de Buochberg, M., Jean-Pierre, H., Carrière, C. & Jumas-Bilak, E. ( 2003b; ). Intra-chromosomal heterogeneity between the four 16S rRNA gene copies in the genus Veillonella: implications for phylogeny and taxonomy. Microbiology 149, 1493–1501.[CrossRef]
    [Google Scholar]
  22. Moore, W. E. C. & Holdeman, L. V. ( 1974; ). Human fecal flora: the normal flora of 20 Japanese-Hawaiians. Appl Microbiol 27, 961–979.
    [Google Scholar]
  23. Morgenstern, B. ( 2002; ). A simple and space-efficient fragment-chaining algorithm for alignment of DNA and protein sequences. Appl Math Lett 15, 11–16.[CrossRef]
    [Google Scholar]
  24. Nakashima, M., Ikoma, H., Yokoyama, M., Suzuki, T. & Sugihara, S. ( 1983; ). Cefotiam passage into the maxillary sinus tissues. Jpn J Antibiot 36, 2730–2732.
    [Google Scholar]
  25. Olsen, G. J., Matsuda, H., Hagstrom, R. & Overbeek, R. ( 1994; ). fastDNAml: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci 10, 41–48.
    [Google Scholar]
  26. Peraino, V. A., Cross, S. A. & Goldstein, E. J. C. ( 1993; ). Incidence and significance of anaerobic bacteremia in a community hospital. Clin Infect Dis 16, S288–S291.[CrossRef]
    [Google Scholar]
  27. Rogosa, M. ( 1969; ). Acidaminococcus gen. n., Acidaminococcus fermentans sp. n., anaerobic Gram-negative diplococci using amino acids as the sole energy source for growth. J Bacteriol 98, 756–766.
    [Google Scholar]
  28. Rogosa, M. ( 1984; ). Anaerobic Gram-negative cocci. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 680–685. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  29. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  30. Sugihara, P. T., Sutter, V. L., Attebery, H. R., Bricknell, K. S. & Finegold, S. M. ( 1974; ). Isolation of Acidaminococcus fermentans and Megasphaera elsdenii from normal human feces. Appl Microbiol 27, 274–275.
    [Google Scholar]
  31. Teyssier, C., Marchandin, H., Siméon de Buochberg, M., Ramuz, M. & Jumas-Bilak, E. ( 2003; ). Atypical 16S rRNA gene copies in Ochrobactrum intermedium strains reveal a large genomic rearrangement by recombination between rrn copies. J Bacteriol 185, 2901–2909.[CrossRef]
    [Google Scholar]
  32. Willems, A. & Collins, M. D. ( 1995; ). Phylogenetic placement of Dialister pneumosintes (formerly Bacteroides pneumosintes) within the Sporomusa subbranch of the Clostridium subphylum of the gram-positive bacteria. Int J Syst Bacteriol 45, 403–405.[CrossRef]
    [Google Scholar]
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