@article{mbs:/content/journal/ijsem/10.1099/ijs.0.64882-0, author = "Takii, Susumu and Hanada, Satoshi and Tamaki, Hideyuki and Ueno, Yutaka and Sekiguchi, Yuji and Ibe, Akihiro and Matsuura, Katsumi", title = "Dethiosulfatibacter aminovorans gen. nov., sp. nov., a novel thiosulfate-reducing bacterium isolated from coastal marine sediment via sulfate-reducing enrichment with Casamino acids ", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2007", volume = "57", number = "10", pages = "2320-2326", doi = "https://doi.org/10.1099/ijs.0.64882-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64882-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "SRB, sulfate-reducing bacteria", abstract = "A sulfate-reducing enrichment culture originating from coastal marine sediment of the eutrophic Tokyo Bay, Japan, was successfully established with Casamino acids as a substrate. A thiosulfate reducer, strain C/G2T, was isolated from the enrichment culture after further enrichment with glutamate. Cells of strain C/G2T were non-motile rods (0.6–0.8 μm×2.2–4.8 μm) and were found singly or in pairs and sometimes in short chains. Spores were not formed. Cells of strain C/G2T stained Gram-negatively, despite possessing Gram-positive cell walls. The optimum temperature for growth was 28–30 °C, the optimum pH was around 7.8 and the optimum salt concentration was 20–30 g l−1. Lactate, pyruvate, serine, cysteine, threonine, glutamate, histidine, lysine, arginine, Casamino acids, peptone and yeast extract were fermented as single substrates and no sugar was used as a fermentative substrate. A Stickland reaction was observed with some pairs of amino acids. Fumarate, alanine, proline, phenylalanine, tryptophan, glutamine and aspartate were utilized only in the presence of thiosulfate. Strain C/G2T fermented glutamate to H2, CO2, acetate and propionate. Thiosulfate and elemental sulfur were reduced to sulfide. Sulfate, sulfite and nitrate were not utilized as electron acceptors. The growth of strain C/G2T on Casamino acids or glutamate was enhanced by co-culturing with Desulfovibrio sp. isolated from the original mixed culture enriched with Casamino acids. The DNA G+C content of strain C/G2T was 41.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain C/G2T formed a distinct cluster with species of the genus Sedimentibacter. The closest relative was Sedimentibacter hydroxybenzoicus (with a gene sequence similarity of 91 %). On the basis of its phylogenetic and phenotypic properties, strain C/G2T (=JCM 13356T=NBRC 101112T=DSM 17477T) is proposed as representing a new genus and novel species, Dethiosulfatibacter aminovorans gen. nov., sp. nov.", }