1887

Abstract

A sulfate-reducing enrichment culture originating from coastal marine sediment of the eutrophic Tokyo Bay, Japan, was successfully established with Casamino acids as a substrate. A thiosulfate reducer, strain C/G2, was isolated from the enrichment culture after further enrichment with glutamate. Cells of strain C/G2 were non-motile rods (0.6–0.8 μm×2.2–4.8 μm) and were found singly or in pairs and sometimes in short chains. Spores were not formed. Cells of strain C/G2 stained Gram-negatively, despite possessing Gram-positive cell walls. The optimum temperature for growth was 28–30 °C, the optimum pH was around 7.8 and the optimum salt concentration was 20–30 g l. Lactate, pyruvate, serine, cysteine, threonine, glutamate, histidine, lysine, arginine, Casamino acids, peptone and yeast extract were fermented as single substrates and no sugar was used as a fermentative substrate. A Stickland reaction was observed with some pairs of amino acids. Fumarate, alanine, proline, phenylalanine, tryptophan, glutamine and aspartate were utilized only in the presence of thiosulfate. Strain C/G2 fermented glutamate to H, CO, acetate and propionate. Thiosulfate and elemental sulfur were reduced to sulfide. Sulfate, sulfite and nitrate were not utilized as electron acceptors. The growth of strain C/G2 on Casamino acids or glutamate was enhanced by co-culturing with sp. isolated from the original mixed culture enriched with Casamino acids. The DNA G+C content of strain C/G2 was 41.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain C/G2 formed a distinct cluster with species of the genus . The closest relative was (with a gene sequence similarity of 91 %). On the basis of its phylogenetic and phenotypic properties, strain C/G2 (=JCM 13356=NBRC 101112=DSM 17477) is proposed as representing a new genus and novel species, gen. nov., sp. nov.

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2007-10-01
2024-12-02
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