Two coccoid phototrophic purple sulfur bacteria were isolated from marine habitats (marine aquaculture pond near Bheemli, Visakhapatnam and marine tidal waters from a fishing harbour, Kakinada) in a medium that contained 3 % NaCl (w/v). Strains JA132 and JA130 are Gram-negative, motile cocci with a single flagellum. Both have an obligate requirement for NaCl. Intracellular photosynthetic membranes are of the vesicular type. Bacteriochlorophyll and most probably carotenoids of the spirilloxanthin series were present as photosynthetic pigments. Both strains were able to grow photolithoautotrophically and photolithoheterotrophically. Chemotrophic and fermentative growth could not be demonstrated. There is no vitamin requirement for strain JA132, while strain JA130 requires niacin, biotin and pantothenate as growth factors. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that both strains cluster with species of the genus belonging to the . The DNA G+C contents of strains JA132 and JA130 were 65.5 and 57.5 mol%, respectively. Based on the 16S rRNA gene sequence analysis, morphological and physiological characteristics, strains JA132 and JA130 are significantly different from each other and from other species of the genus and are recognized as two novel species, for which the names sp. nov. and sp. nov. are proposed. The type strains of sp. nov. and sp. nov. are JA132 (=MTCC 8120=ATCC BAA-1362=JCM 14149=DSM 18805) and JA130 (=ATCC BAA-1353=DSM 18858=JCM 14150), respectively.


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  1. Anil Kumar, P. A., Sasi Jyothsna, T. S., Srinivas, T. N. R., Sasikala, Ch., Ramana, Ch. V. & Imhoff, J. F. ( 2007; ). Marichromatium bheemlicum sp. nov., a non-diazotrophic photosynthetic gammaproteobacterium from a marine aquaculture pond. Int J Syst Evol Microbiol 57, 1261–1265.[CrossRef]
    [Google Scholar]
  2. Felsenstein, J. ( 1989; ). phylip (Phylogenetic inference package) version 3.5.1. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  3. Guyoneaud, R., Matheron, R., Liesack, W., Imhoff, J. F. & Caumette, P. ( 1997; ). Thiorhodococcus minus, gen. nov., sp. nov., a new purple sulfur bacterium isolated from coastal lagoon sediments. Arch Microbiol 168, 16–23.[CrossRef]
    [Google Scholar]
  4. Imhoff, J. F. ( 2001; ). True marine and halophilic anoxygenic phototrophic bacteria. Arch Microbiol 176, 243–254.[CrossRef]
    [Google Scholar]
  5. Imhoff, J. F., Süling, J. & Petri, R. ( 1998; ). Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium. Int J Syst Bacteriol 48, 1129–1143.[CrossRef]
    [Google Scholar]
  6. Rabold, S., Gorlenko, V. M. & Imhoff, J. F. ( 2006; ). Thiorhodococcus mannitoliphagus sp. nov., a purple sulfur bacterium from the White Sea. Int J Syst Evol Microbiol 56, 1945–1951.[CrossRef]
    [Google Scholar]
  7. Sanger, F., Nicklen, S. & Coulson, A. R. ( 1977; ). DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci U S A 74, 5463–5467.[CrossRef]
    [Google Scholar]
  8. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1997; ). The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  9. Zaar, A., Fuchs, G., Golecki, J. R. & Overmann, J. ( 2003; ). A new purple sulfur bacterium isolated from a littoral microbial mat, Thiorhodococcus drewsii sp. nov. Arch Microbiol 179, 174–183.
    [Google Scholar]

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vol. , part 11, pp. 2458 - 2461

Phase-contrast micrograph of strains JA132 and JA130

Electron micrograph of negatively stained cells of strains JA132 and JA130

Electron micrograph of ultrathin section of strains JA132 and JA130

Whole-cell absorption spectra (a) and acetone spectra (b) [Single PDF file](164 KB)


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