1887

Abstract

Three pink-pigmented, facultatively methylotrophic strains, designated MP1, MP2 and MR, were isolated from seawater from southern China and characterized. Analysis of their complete 16S rRNA gene sequences revealed that they constituted three separate phylogenetic groups, showing the highest levels of similarity with respect to some members of the genus . PCR amplification also showed the gene coding for the -subunit of methanol dehydrogenase () to be present in all strains, indicating a methylotrophic metabolism. All three strains utilized -fructose, ethanol and nutrient agar as carbon sources, but did not utilize sucrose, citrate, acetate or formaldehyde. On the basis of the phenotypic, phylogenetic and genotypic analyses, strain MR represents a novel species, for which the name sp. nov. is proposed, with MR (=CGMCC 1.6474 =NCCB 100140) as the type strain. Strains MP1 and MP2 respectively represent novel strains of the species and .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64877-0
2007-08-01
2024-11-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/8/1699.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64877-0&mimeType=html&fmt=ahah

References

  1. Anesti V., Vohra J., Goonetilleka S., McDonald I. R., Sträubler B., Stackebrandt E., Kelly D. P., Wood A. P. 2004; Molecular detection and isolation of facultatively methylotrophic bacteria, including Methylobacterium podarium sp. nov., from the human foot microflora. Environ Microbiol 6:820–830 [CrossRef]
    [Google Scholar]
  2. Bodrossy L., Murrell J. C., Dalton H., Kalman M., Puskas L. G., Kovacs K. L. 1995; Heat-tolerant methanotrophic bacteria from the hot water effluent of a natural gas field. Appl Environ Microbiol 61:3549–3555
    [Google Scholar]
  3. Bousfield I. J., Green P. N. 1985; Reclassification of bacteria of the genus Protomonas Urakami and Komagata 1984 in the genus Methylobacterium (Patt, Cole, and Hanson) emend. Green and Bousfield 1983 Int J Syst Bacteriol 35: 209 [CrossRef]
    [Google Scholar]
  4. Doronina N. V., Trotsenko Y. A., Tourova T. P., Kuznetsov B. B., Leisinger T. 2000; Methylopila helvetica sp. nov. and Methylobacterium dichloromethanicum sp. nov., novel aerobic facultatively methylotrophic bacteria utilizing dichloromethane. Syst Appl Microbiol 23:210–218 [CrossRef]
    [Google Scholar]
  5. Doronina N. V., Trotsenko Y. A., Kuznetsov B. B., Tourova T. P., Salkinoja-Salonen M. S. 2002; Methylobacterium suomiense sp. nov. and Methylobacterium lusitanum sp. nov., aerobic, pink-pigmented, facultatively methylotrophic bacteria. Int J Syst Evol Microbiol 52:773–776 [CrossRef]
    [Google Scholar]
  6. Gallego V., García M. T., Ventosa A. 2005a; Methylobacterium hispanicum sp. nov. and Methylobacterium aquaticum sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 55:281–287 [CrossRef]
    [Google Scholar]
  7. Gallego V., García M. T., Ventosa A. 2005b; Methylobacterium variabile sp. nov., a methylotrophic bacterium isolated from an aquatic environment. Int J Syst Evol Microbiol 55:1429–1433 [CrossRef]
    [Google Scholar]
  8. Gallego V., García M. T., Ventosa A. 2005c; Methylobacterium isbiliense sp. nov., isolated from the drinking water system of Sevilla, Spain. Int J Syst Evol Microbiol 55:2333–2337 [CrossRef]
    [Google Scholar]
  9. Gallego V., García M. T., Ventosa A. 2006; Methylobacterium adhaesivum sp. nov., a methylotrophic bacterium isolated from drinking water. Int J Syst Evol Microbiol 56:339–342 [CrossRef]
    [Google Scholar]
  10. Green P. N. 2006; Methylobacterium . In The Prokaryotes. A Handbook on the Biology of Bacteria . , 3rd edn. vol 5 pp 257–265 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
  11. Green P. N., Bousfield I. J. 1981; The taxonomy of pink pigmented facultatively methylotrophic bacteria. In Microbial Growth on C1-Compounds pp 285–293 Edited by Dalton H. London: Heyden & Son;
    [Google Scholar]
  12. Green P. N., Bousfield I. J. 1982; A taxonomic study of some Gram-negative facultatively methylotrophic bacteria. J Gen Microbiol 128:623–638
    [Google Scholar]
  13. Green P. N., Bousfield I. J. 1983; Emendation of Methylobacterium Patt, Cole, and Hanson 1976; Methylobacterium rhodinum (Heumann 1962) comb. nov. corrig.; Methylobacterium radiotolerans (Ito & Iizuka 1971) comb. nov., corrig.; and Methylobacterium mesophilicum (Austin & Goodfellow 1979) comb. nov. Int J Syst Bacteriol 33:875–877 [CrossRef]
    [Google Scholar]
  14. Green P. N., Bousfield I. J., Hood D. 1988; Three new Methylobacterium species: M. rhodesianum sp. nov., M. zatmanii sp. nov., and M. fujisawaense sp. nov. Int J Syst Bacteriol 38:124–127 [CrossRef]
    [Google Scholar]
  15. Heyer J., Galchenko V. F., Dunfield P. F. 2002; Molecular phylogeny of type II methane-oxidizing bacteria isolated from various environments. Microbiology 148:2831–2846
    [Google Scholar]
  16. Hiraishi A., Furuhata K., Matsumoto A., Koike K. A., Fukuyama M., Tabuchi K. 1995; Phenotypic and genetic diversity of chlorine-resistant Methylobacterium strains isolated from various environments. Appl Environ Microbiol 61:2099–2107
    [Google Scholar]
  17. Jourand P., Giraud E., Be'na G., Sy A., Willems A., Gillis M., Dreyfus B., de Lajudie P. 2004; Methylobacterium nodulans sp. nov., for a group of aerobic, facultatively methylotrophic, legume root-nodule-forming and nitrogen-fixing bacteria. Int J Syst Evol Microbiol 54:2269–2273 [CrossRef]
    [Google Scholar]
  18. Lee C.-H., Tang Y. F., Lin J. W. 2004; Underdiagnosis of urinary tract infection caused by Methylobacterium species with current standard processing of urine culture and its clinical implications. J Med Microbiol 53:755–759 [CrossRef]
    [Google Scholar]
  19. Madhaiyan M., Kim B. Y., Poonguzhali S., Kwon S. W., Song M. H., Ryu J. H., Go S. J., Koo B. S., Sa T. M. 2007; Methylobacterium oryzae sp. nov., an aerobic, pink-pigmented, facultatively methylotrophic, 1-aminocyclopropane-1-carboxylate deaminase-producing bacterium isolated from rice. Int J Syst Evol Microbiol 57:326–331 [CrossRef]
    [Google Scholar]
  20. McDonald I. R., Doronina N. V., Trotsenko Y. A., McAnulla C., Murrell J. C. 2001; Hyphomicrobium chloromethanicum sp. nov. and Methylobacterium chloromethanicum sp. nov., chloromethane-utilizing bacteria isolated from a polluted environment. Int J Syst Evol Microbiol 51:119–122
    [Google Scholar]
  21. Möller E. M., Bahnweg G., Sandermann H., Geiger H. H. 1992; A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Res 20:6115–6116 [CrossRef]
    [Google Scholar]
  22. Patt T. E., Cole G. C., Hanson R. S. 1976; Methylobacterium , a new genus of facultatively methylotrophic bacteria. Int J Syst Bacteriol 26:226–229 [CrossRef]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Urakami T., Araki H., Suzuki K., Komogata K. 1993; Further studies of the genus Methylobacterium and description of Methylobacterium aminovorans sp. nov. Int J Syst Bacteriol 43:504–513 [CrossRef]
    [Google Scholar]
  25. Van Aken B., Peres C. M., Doty S. L., Yoon J. M., Schnoor J. L. 2004; Methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees ( Populus deltoides × nigra DN34. Int J Syst Evol Microbiol 54:1191–1196 [CrossRef]
    [Google Scholar]
  26. Wood A. P., Kelly D. P., McDonald I. R., Jordan S. L., Morgan T. D., Khan S., Murrell J. C., Borodina E. 1998; A novel pink-pigmented facultative methylotroph, Methylobacterium thiocyanatum sp. nov., capable of growth on thiocyanate or cyanate as sole nitrogen sources. Arch Microbiol 169:148–158 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.64877-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64877-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error