1887

Abstract

Fifteen strains of moderately halophilic, Gram-positive cocci were isolated from a traditional fermented shrimp paste (‘ka-pi’) produced in Thailand. These bacteria were strictly aerobic, non-motile, non-sporulating and catalase- and oxidase-positive. They produced orange pigment and grew in the presence of 1.5–25 % (w/v) NaCl. They grew optimally in 10 % (w/v) NaCl, at pH 8.5 and at 37 °C. The cell-wall peptidoglycan was of -Lys type. Menaquinone with six isoprene units (MK-6) was a major component. The dominant cellular fatty acids were anteiso-C and iso-C. DNA G+C contents were in the range 44.5–47.5 mol%. Comparative 16S rRNA gene sequence analyses indicated that representative strain PN1-2 was related most closely to JCM 14630, with 97.3 % similarity. The other novel strains were included in the same species based on their levels of DNA–DNA relatedness to strain PN1-2 (≥76.6 %) but showed low DNA–DNA relatedness to JCM 14630 (21.7 %). On the basis of the phenotypic and molecular data presented, the 15 novel strains are suggested to represent a single novel species of the genus , for which the name sp. nov. is proposed. The type strain is PN1-2 (=JCM 12822 =PCU 242 =TISTR 1562).

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2007-09-01
2021-04-11
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