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Abstract

Fifteen strains of moderately halophilic, Gram-positive cocci were isolated from a traditional fermented shrimp paste (‘ka-pi’) produced in Thailand. These bacteria were strictly aerobic, non-motile, non-sporulating and catalase- and oxidase-positive. They produced orange pigment and grew in the presence of 1.5–25 % (w/v) NaCl. They grew optimally in 10 % (w/v) NaCl, at pH 8.5 and at 37 °C. The cell-wall peptidoglycan was of -Lys type. Menaquinone with six isoprene units (MK-6) was a major component. The dominant cellular fatty acids were anteiso-C and iso-C. DNA G+C contents were in the range 44.5–47.5 mol%. Comparative 16S rRNA gene sequence analyses indicated that representative strain PN1-2 was related most closely to JCM 14630, with 97.3 % similarity. The other novel strains were included in the same species based on their levels of DNA–DNA relatedness to strain PN1-2 (≥76.6 %) but showed low DNA–DNA relatedness to JCM 14630 (21.7 %). On the basis of the phenotypic and molecular data presented, the 15 novel strains are suggested to represent a single novel species of the genus , for which the name sp. nov. is proposed. The type strain is PN1-2 (=JCM 12822 =PCU 242 =TISTR 1562).

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2007-09-01
2019-10-13
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References

  1. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  2. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Forbes, L. ( 1981; ). Rapid flagella stain. J Clin Microbiol 13, 807–809.
    [Google Scholar]
  5. França, L., Rainey, F. A., Nobre, M. F. & da Costa, M. S. ( 2006; ). Salinicoccus salsiraiae sp. nov.: a new moderately halophilic gram-positive bacterium isolated from salted skate. Extremophiles 10, 531–536.[CrossRef]
    [Google Scholar]
  6. Hao, M. V., Kocur, M. & Komagata, K. ( 1984; ). Marinococcus gen. nov., a new genus for motile cocci with meso-diaminopimelic acid in the cell wall; and Marinococcus albus sp. nov. and Marinococcus halophilus (Novitsky and Kushner) comb. nov. J Gen Appl Microbiol 30, 449–459.[CrossRef]
    [Google Scholar]
  7. Hucker, G. J. & Conn, H. J. ( 1923; ). Method of Gram staining. N Y State Agric Exp Stn Tech Bull 93, 3–37.
    [Google Scholar]
  8. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  9. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  10. Kumar, S., Tamura, K., Jakobson, I.-B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetic analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  11. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  12. Li, W.-J., Schumann, P., Zhang, Y.-Q., Chen, G. Z., Tian, X.-P., Xu, L.-H., Stackebrandt, E. & Jiang, C.-L. ( 2005; ). Marinococcus halotolerans sp. nov., isolated from Qinghai, north-west China. Int J Syst Evol Microbiol 55, 1801–1804.[CrossRef]
    [Google Scholar]
  13. Márquez, M. C., Ventosa, A. & Ruíz-Berraquero, F. ( 1990; ). Marinococcus hispanicus, a new species of moderately halophilic gram-positive cocci. Int J Syst Bacteriol 40, 165–169.[CrossRef]
    [Google Scholar]
  14. Saito, H. & Miura, K. I. ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef]
    [Google Scholar]
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  17. Satomi, M., Kimura, B., Mizoi, M., Sato, T. & Fujii, T. ( 1997; ). Tetragenococcus muriaticus sp. nov., a new moderately halophilic lactic acid bacterium isolated from fermented squid liver sauce. Int J Syst Bacteriol 47, 832–836.[CrossRef]
    [Google Scholar]
  18. Seearunruangchai, A., Tanasupawat, S., Keeratipibul, S., Thawai, C., Itoh, T. & Yamada, Y. ( 2004; ). Identification of acetic acid bacteria isolated from fruits and related materials collected in Thailand. J Gen Appl Microbiol 50, 47–53.[CrossRef]
    [Google Scholar]
  19. Spring, S., Ludwig, W., Marquez, M. C., Ventosa, A. & Schleifer, K.-H. ( 1996; ). Halobacillus gen. nov., with description of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophila comb. nov. Int J Syst Bacteriol 46, 492–496.[CrossRef]
    [Google Scholar]
  20. Stackebrandt, E., Koch, C., Gvozdiak, O. & Schumann, P. ( 1995; ). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45, 682–692.[CrossRef]
    [Google Scholar]
  21. Tamaoka, J. S. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  22. Tanasupawat, S., Okada, S., Suzuki, K., Kozaki, M. & Komagata, K. ( 1993; ). Lactic acid bacteria, particularly heterofermentative lactobacilli, found in fermented foods in Thailand. Bull JFCC 9, 65–78.
    [Google Scholar]
  23. Tanasupawat, S., Shida, O., Okada, S. & Komagata, K. ( 2000; ). Lactobacillus acidipiscis sp. nov. and Weissella thailandensis sp. nov., isolated from fermented fish in Thailand. Int J Syst Evol Microbiol 50, 1479–1485.[CrossRef]
    [Google Scholar]
  24. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  25. Thornley, M. J. ( 1960; ). The differentiation of Pseudomonas from other Gram-negative bacteria on the basis of arginine metabolism. J Appl Bacteriol 23, 37–52.[CrossRef]
    [Google Scholar]
  26. Ventosa, A., Marquez, M. C., Ruiz-Berraquero, F. & Kocur, M. ( 1990; ). Salinicoccus roseus gen. nov., sp. nov., a new moderately halophilic Gram-positive coccus. Syst Appl Microbiol 13, 29–33.[CrossRef]
    [Google Scholar]
  27. Ventosa, A., Marquez, M. C., Weiss, N. & Tindall, B. J. ( 1992; ). Transfer of Marinococcus hispanicus to the genus Salinicoccus as Salinicoccus hispanicus comb. nov. Syst Appl Microbiol 15, 530–534.[CrossRef]
    [Google Scholar]
  28. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  29. Yoon, J.-H., Lee, K.-C., Weiss, N., Kang, K. H. & Park, Y.-H. ( 2003; ). Jeotgalicoccus halotolerans gen. nov., sp. nov. and Jeotgalicoccus psychrophilus sp. nov., isolated from the traditional Korean fermented seafood jeotgal. Int J Syst Evol Microbiol 53, 595–602.[CrossRef]
    [Google Scholar]
  30. Zhang, W., Xue, Y., Ma, Y., Zhou, P., Ventosa, A. & Grant, W. D. ( 2002; ). Salinicoccus alkaliphilus sp. nov., a novel alkaliphile and moderate halophile from Baer Soda Lake in Inner Mongolia Autonomous Region, China. Int J Syst Evol Microbiol 52, 789–793.[CrossRef]
    [Google Scholar]
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Scanning electron micrograph of cells of strain PN1-2 grown on JCM medium no. 377 at 37 °C for 5 days. Bar, 1 µm.

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Colonies of strain PN1-2 grown on JCM medium no. 377 medium at 37 °C for 5 days.

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Maximum-parsimony phylogenetic tree showing the relationships between sp. nov. strains PN1-2 and PN7-1 and related species, based on 16S rRNA gene sequences. [PDF](17 KB)

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DNA G+C content and DNA–DNA relatedness among the novel strains and JCM 14630 . [PDF](14 KB)

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