Two novel bacterial strains were isolated from a paddy soil sample collected in Japan using GPM agar plates supplemented with superoxide dismutase and/or catalase. The strains were Gram-positive, catalase-positive and motile, with lysine as the peptidoglycan diagnostic diamino acid and acetyl as the peptidoglycan acyl type. The major menaquinone was MK-9(H). Mycolic acids were not detected. The G+C content of the DNA was 66–68 mol%. On the basis of phenotypic analysis, 16S rRNA gene sequence comparisons and DNA–DNA hybridization data, it is proposed that these strains represent two novel species, sp. nov. (type strain is KV-651=NRRL B-24478=NBRC 102055) and sp. nov. (type strain is KV-653=NRRL B-24479=NBRC 102056), respectively.


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Similarity values of 16S rRNA gene sequences and DNA-DNA relatedness values for the two isolated strains and related species. [ PDF] (50 KB)



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