1887

Abstract

A novel actinobacterium, designated strain SST-39, was isolated from a marine sediment sample collected in Jeju, Republic of Korea. Cells were facultatively anaerobic, Gram-positive, non-endospore-forming, non-motile rods. Colonies were circular, slightly convex, opaque and brilliant yellow. The cell-wall peptidoglycan of the organism contained -diaminopimelic acid as the diagnostic diamino acid. Polar lipids included phosphatidylglycerol and diphosphatidylglycerol. The predominant menaquinone was MK-9(H). The major cellular fatty acids were anteiso-C, C and C. The DNA G+C content was 68.4 mol%. A phylogenetic tree based on 16S rRNA gene sequences indicated that the isolate belonged to the family and formed a unique cluster with the type strain of (97.0 % sequence similarity). Other phylogenetic neighbours were the type strains of (95.1 % 16S rRNA gene sequence similarity) and (95.1 %). On the basis of its phenotypic and phylogenetic distinctiveness, strain SST-39 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SST-39 (=DSM 18582 =KCTC 19196).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64868-0
2008-04-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/4/785.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64868-0&mimeType=html&fmt=ahah

References

  1. Collins, M. D. ( 1985; ). Analysis of isoprenoid quinones. Methods Microbiol 18, 329–366.
    [Google Scholar]
  2. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Department of Genome Sciences, University of Washington, Seattle, USA.
  5. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  6. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P., Ward, J. M. & Schrempf, H. ( 1985; ). Genetic Manipulation of Streptomyces. A Laboratory Manual. Norwich: John Innes Foundation.
  7. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  8. Kroppenstedt, R. M. ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics, pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  9. Lee, S. D. ( 2006; ). Kineococcus marinus sp. nov., isolated from a marine sediment of the coast of Jeju, Korea. Int J Syst Evol Microbiol 56, 1279–1283.[CrossRef]
    [Google Scholar]
  10. Maszenan, A. M., Seviour, R. J., Patel, B. K., Schumann, P. & Rees, G. N. ( 1999; ). Tessaracoccus bendigoensis gen. nov., sp. nov., a Gram-positive coccus occurring in regular packages or tetrads, isolated from activated sludge biomass. Int J Syst Bacteriol 49, 459–468.[CrossRef]
    [Google Scholar]
  11. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  12. Minnikin, D. E., Alshamaony, L. & Goodfellow, M. ( 1977; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin layer chromatographic analysis of whole-cell methanolysates. J Gen Microbiol 88, 200–204.
    [Google Scholar]
  13. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  14. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  15. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  16. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  17. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64868-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64868-0
Loading

Data & Media loading...

Supplements

Morphological features of strain SST-39 observed by transmission electron microscopy. The culture was grown on TSA at 30 °C for 3 days. Bar, 0.5 µm.

IMAGE

Neighbour-joining tree based on 16S rRNA gene sequences showing the relationships between strain SST-39 and representatives of the suborder and related taxa. [PDF](19 KB)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error