gen. nov., sp. nov., of the phylum , isolated from marine sediment Free

Abstract

A novel bacterium designated Mok-1-85 was isolated from a marine sediment sample collected from Okinawa Island, Japan. Cells of strain Mok-1-85 stained Gram-negative, were catalase- and oxidase-positive and were non-motile. In a neighbour-joining tree based on 16S rRNA gene sequences, the novel strain clustered with the genus , a member of the family ‘’. The novel isolate shared low 16S rRNA gene sequence similarities (≤86 %) with the members of the genus and other related taxa. The major isoprenoid quinone was MK-7 and the predominant fatty acids of this organism were iso-C, C 5 and C 3-OH. The G+C content of the DNA was 38 mol%. Combined phylogenetic and physiological data showed that strain Mok-1-85 represents a novel genus and species for which the name gen. nov., sp. nov. is proposed. The type strain is Mok-1-85 (=NBRC 101625=KCTC 12970=CIP 109411).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64854-0
2007-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/8/1689.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64854-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Bäckhed F., Ley R. E., Sonnenburg J. L., Peterson D. A., Gordon J. I. 2005; Host-bacterial mutualism in the human intestine. Science 307:1915–1920 [CrossRef]
    [Google Scholar]
  3. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  4. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1993 Manual for the Identification of Medical Bacteria , 3rd edn. London: Cambridge University Press;
    [Google Scholar]
  6. Fautz E., Reichenbach H. 1980; A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91 [CrossRef]
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  8. Garrity G. M., Holt J. G. 2001; The road map to the Manual . In Bergey's Manual of Systematic Bacteriology . , 2nd edn. vol 1 pp 119–166 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
  9. Hiraishi A., Shin Y. K., Ueda Y., Sugiyama J. 1994; Automated sequencing of PCR-amplified 16S rDNA on ‘Hydrolink’ gels. J Microbiol Methods 19:145–154 [CrossRef]
    [Google Scholar]
  10. Hudson J. A., Schofield K. M., Morgan H. W., Daniel R. M. 1989; Thermonema lapsum gen. nov., sp. nov., a thermophilic gliding bacterium. Int J Syst Bacteriol 39:485–487 [CrossRef]
    [Google Scholar]
  11. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram negative bacteria. J Bacteriol 66:24–26
    [Google Scholar]
  12. Leser T. D., Amenuvor J. Z., Jensen T. K., Lindecrona R. H., Boye M., Møller K. 2002; Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited. Appl Environ Microbiol 68:673–690 [CrossRef]
    [Google Scholar]
  13. Lewin R. A. 1970; Flexithrix dorotheae gen. et sp. nov. ( Flexibacterales ); and suggestions for reclassifying sheathed bacteria. Can J Microbiol 16:511–515 [CrossRef]
    [Google Scholar]
  14. Lewin R. A., Lounsbery D. C. 1969; Isolation, cultivation and characterization of flexibacteria. J Gen Microbiol 58:145–170 [CrossRef]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  16. Minamisawa K. 1990; Division of rhizobitoxine-producing and hydrogen-uptake positive strains of Bradyrhizobium japonicum by nifDKE sequence divergence. Plant Cell Physiol 31:81–89
    [Google Scholar]
  17. Nakagawa Y., Yamasato K. 1993; Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 139:1155–1161 [CrossRef]
    [Google Scholar]
  18. Nakagawa Y., Hamana K., Sakane T., Yamasato K. 1997 Reclassification of Cytophaga aprica (Lewin 1969) Reichenbach 1989 in Flammeovirga gen. nov., as Flammeovirga aprica comb.nov. and of Cytophaga diffluens (ex Stanier 1940; emend. Lewin 1969) Reichenbach 1989 in Persicobacter gen. nov. as Persicobacter diffluens comb. nov. Int J Syst Bacteriol 47, 220–223 [CrossRef]
  19. Perry L. B. 1973; Gliding motility in some non-spreading flexibacteria. J Appl Bacteriol 36:227–232 [CrossRef]
    [Google Scholar]
  20. Reichenbach H. 1989; Genus I. Cytophaga. pp 2015–2050 In Bergey's Manual of Systematic Bacteriology vol 3 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore, MD: The Williams & Wilkins Co;
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Schmidt K., Connor A., Britton G. 1994; Analysis of pigments: carotenoids and related polyenes. In Chemical Methods in Prokaryotic Systematics pp 403–461 Edited by Goodfellow M., O'Donnell A. G. West Sussex: John Wiley & Sons;
    [Google Scholar]
  23. Shewan J. M., McMeekin T. A. 1983; Taxonomy (and ecology) of Flavobacterium and related genera. Annu Rev Microbiol 37:233–252 [CrossRef]
    [Google Scholar]
  24. Smibert R. M., Krieg N. R. 1981; General characterization. In Manual of Methods for General Bacteriology . pp 409–443 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
  25. Swofford D. L. 2000 paup*: Phylogenetic analysis using parsimony (*and other methods), version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  26. Takahashi M., Suzuki K.-I., Nakagawa Y. 2006 Emendation of the genus Flammeovirga and Flammeovirga aprica with the proposal of Flammeovirga arenaria nom.rev., comb. nov., and Flammeovirga yaeyamensis sp. nov. Int J Syst Evol Microbiol 562095–2100 [CrossRef]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64854-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64854-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed