1887

Abstract

New diseases known locally as ‘hoja de perejil’ of tomato ( Mill) and ‘brotes grandes’ of potato ( L.) were first recognized in surveys of production fields in Bolivia during 2000–2003. Alfalfa () witches' broom and little leaf diseases of native weeds and mora-mora () were also identified near to production fields. Phytoplasma aetiology was attributed to each of these diseases following detection and initial identification of aster yellows group (16SrI) phytoplasmas in all five diseased plant species. While potato, alfalfa and mora-mora plants contained indistinguishable 16SrI-B strains, ‘hoja de perejil’ (THP) and morrenia little leaf (MVLL)-associated phytoplasma strains shared 97.5 % 16S rRNA gene sequence similarity with ‘ Phytoplasma asteris’ and related strains and <95 % similarity with all other ‘ Phytoplasma’ species. Phylogenetic analysis of 16S rRNA gene sequences indicated that the THP and MVLL phytoplasmas represent a novel lineage within the aster yellows (16SrI) group and, on the basis of unique 16S rRNA gene sequences, we propose that THP and MVLL phytoplasmas represent ‘ Phytoplasma lycopersici’, with THP as the reference strain.

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2007-08-01
2019-10-18
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Supplements

vol. , part 8, pp. 1704 - 1710

Acronyms and abbreviations, strain designations and RFLP groups of phytoplasma sequences used to construct the phylogenetic tree.

Similarity coefficients derived from RFLPs based on putative restriction site analysis of nucleotide sequences of phytoplasma 16S rRNA genes of THP, MVLL, PBG, AlfWB and MMLL and other selected phytoplasmas

Sequence similarities derived from BLAST comparisons of the 16S rRNA gene sequence of THP with that of PBG and other selected phytoplasmas

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