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Abstract

A novel thermophilic, hydrogen-oxidizing bacterium, designated strain CP.B2, was isolated from a terrestrial hot spring in Waiotapu, New Zealand. Cells were motile, slightly rod-shaped, non-spore-forming and Gram-negative. Isolate CP.B2 was an obligate chemolithotroph, growing by utilizing H as electron donor and O as corresponding electron acceptor. Elemental sulfur (S) or thiosulfate () was essential for growth. Microbial growth occurred under microaerophilic conditions in 1.0–10.0 % (v/v) O [optimum 4–8 % (v/v) O], between 45 and 75 °C (optimum 70 °C) and at pH values of 4.8–5.8 (optimum pH 5.4). The DNA G+C content was 29.3 mol%. 16S rRNA gene sequence analysis demonstrated that strain CP.B2 belonged to the order , with a close phylogenetic relationship to (94 % sequence similarity to the type strain). However, genotypic and metabolic characteristics differentiated the novel isolate from previously described genera of the . Therefore, CP.B2 represents a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is CP.B2 (=JCM 14244 =DSM 18763).

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2008-02-01
2024-03-28
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References

  1. Aguiar, P., Beveridge, T. J. & Reysenbach, A.-L.(2004).Sulfurihydrogenibium azorense sp. nov., a thermophilic hydrogen-oxidizing microaerophile from terrestrial hot springs in the Azores. Int J Syst Evol Microbiol 54, 33–39.[CrossRef] [Google Scholar]
  2. Cline, J. D.(1969). Spectrophotometric determination of hydrogen sulfide in natural waters. Limnol Oceanogr 14, 454–458.[CrossRef] [Google Scholar]
  3. Dempster, E. L., Pryor, K. V., Francis, D., Young, J. E. & Rogers, H. J.(1999). Rapid DNA extraction from ferns for PCR-based analyses. Biotechniques 27, 66–68. [Google Scholar]
  4. Ellis, D. G., Bizzoco, R. L. W., Maezato, Y., Baggett, J. N. & Kelley, S. T.(2005). Microscopic examination of acidic hot springs of Waiotapu, North Island, New Zealand. N Z J Mar Freshw Res 39, 1001–1011.[CrossRef] [Google Scholar]
  5. Euzéby, J. P.(1997). List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47, 590–592.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1993).phylip (phylogeny inference package) version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  7. Gilboa-Garber, N.(1971). Direct spectrophotometric determination of inorganic sulfide in biological materials and in other complex mixtures. Anal Biochem 43, 129–133.[CrossRef] [Google Scholar]
  8. Götz, D., Banta, A., Beveridge, T. J., Rushdi, A. I., Simoneit, B. R. T. & Reysenbach, A.(2002).Persephonella marina gen. nov., sp. nov. and Persephonella guaymasensis sp. nov., two novel, thermophilic, hydrogen-oxidizing microaerophiles from deep-sea hydrothermal vents. Int J Syst Evol Microbiol 52, 1349–1359.[CrossRef] [Google Scholar]
  9. Griffiths, E. & Gupta, R. S.(2006). Molecular signatures in protein sequences that are characteristics of the phylum Aquificae. Int J Syst Evol Microbiol 56, 99–107.[CrossRef] [Google Scholar]
  10. Hedenquist, J. W.(1986). Geothermal systems in the Taupo Volcanic Zone: their characteristics and relation to volcanism and mineralisation. In Late Cenozoic Volcanism, Royal Society of New Zealand Bulletin 23, pp. 134–168. Edited by I. E. M. Smith. Wellington: Royal Society of New Zealand.
  11. Hetzer, A., Morgan, H. W., McDonald, I. R. & Daughney, C. J.(2007). Microbial life in Champagne Pool, a geothermal spring in Waiotapu, New Zealand. Extremophiles 11, 605–614.[CrossRef] [Google Scholar]
  12. Huber, R. & Eder, W.(2006).Aquificales. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 925–928. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  13. Huber, R., Wilharm, T., Huber, D., Trincone, A., Burggraf, S., Koenig, H., Rachel, R., Rockinger, I., Fricke, H. & Stetter, K. O.(1992).Aquifex pyrophilus gen. nov., sp. nov., represents a novel group of marine hyperthermophilic hydrogen-oxidizing bacteria. Syst Appl Microbiol 15, 340–351.[CrossRef] [Google Scholar]
  14. Huber, R., Eder, W., Heldwein, S., Wanner, G., Huber, H., Rachel, R. & Stetter, K. O.(1998).Thermocrinis ruber gen. nov., sp. nov., a pink-filament-forming hyperthermophilic bacterium isolated from Yellowstone National Park. Appl Environ Microbiol 64, 3576–3583. [Google Scholar]
  15. Huber, H., Diller, S., Horn, C. & Rachel, R.(2002).Thermovibrio ruber gen. nov., sp. nov., an extremely thermophilic, chemolithoautotrophic, nitrate-reducing bacterium that forms a deep branch within the phylum Aquificae. Int J Syst Evol Microbiol 52, 1859–1865.[CrossRef] [Google Scholar]
  16. Hucker, G. J. & Conn, H. J.(1927). Further studies on the methods of Gram staining. N Y State Agric Exp Stn Tech Bull 128, 1–34. [Google Scholar]
  17. Johnson, J. L.(1994). Similarity analysis of rRNAs. In Methods for General and Molecular Bacteriology, pp. 683–700. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  18. Jones, B., Renaut, R. W. & Rosen, M. R.(2001). Biogenicity of gold- and silver-bearing siliceous sinters forming in hot (75 °C) anaerobic spring-waters of Champagne Pool, Waiotapu, North Island, New Zealand. J Geol Soc 158, 895–911.[CrossRef] [Google Scholar]
  19. Kawasumi, T., Igarashi, Y., Kodama, T. & Minoda, Y.(1984).Hydrogenobacter thermophilus gen. nov., sp. nov., an extremely thermophilic, aerobic, hydrogen-oxidizing bacterium. Int J Syst Bacteriol 34, 5–10.[CrossRef] [Google Scholar]
  20. L'Haridon, S., Cilia, V., Messner, P., Raguénès, G., Gambacorta, A., Sleytr, U. B., Prieur, D. & Jeanthon, C.(1998).Desulfurobacterium thermolithotrophum gen. nov., sp. nov., a novel autotrophic, sulphur-reducing bacterium isolated from a deep-sea hydrothermal vent. Int J Syst Bacteriol 48, 701–711.[CrossRef] [Google Scholar]
  21. Lloyd, E. F.(1959). The hot springs and hydrothermal eruptions of Waiotapu. N Z J Geol Geophys 2, 141–176.[CrossRef] [Google Scholar]
  22. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors(2004).arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  23. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  24. Mountain, B. W., Benning, L. G. & Boerema, J. A.(2003). Experimental studies on New Zealand hot spring sinters: rates of growth and textural development. Can J Earth Sci 40, 1643–1667.[CrossRef] [Google Scholar]
  25. Nakagawa, S., Nakamura, S., Inagaki, F., Takai, K., Shirai, N. & Sako, Y.(2004).Hydrogenivirga caldilitoris gen. nov., sp. nov., a novel extremely thermophilic, hydrogen- and sulfur-oxidizing bacterium from a coastal hydrothermal field. Int J Syst Evol Microbiol 54, 2079–2084.[CrossRef] [Google Scholar]
  26. Nakagawa, S., Shtaih, Z., Banta, A., Beveridge, T. J., Sako, Y. & Reysenbach, A. L.(2005).Sulfurihydrogenibium yellowstonense sp. nov., an extremely thermophilic, facultatively heterotrophic, sulfur-oxidizing bacterium from Yellowstone National Park, and emended descriptions of the genus Sulfurihydrogenibium, Sulfurihydrogenibium subterraneum and Sulfurihydrogenibium azorense. Int J Syst Evol Microbiol 55, 2263–2268.[CrossRef] [Google Scholar]
  27. Phoenix, V. R., Renaut, R. W., Jones, B. & Ferris, F. G.(2005). Bacterial S-layer preservation and rare arsenic-antimony-sulphide bioimmobilization in siliceous sediments from Champagne Pool hot spring, Waiotapu, New Zealand. J Geol Soc 162, 323–331.[CrossRef] [Google Scholar]
  28. Stöhr, R., Waberski, A., Völker, H., Tindall, B. J. & Thomm, M.(2001).Hydrogenothermus marinus gen. nov., sp. nov., a novel thermophilic hydrogen-oxidizing bacterium, recognition of Calderobacterium hydrogenophilum as a member of the genus Hydrogenobacter and proposal of the reclassification of Hydrogenobacter acidophilus as Hydrogenobaculum acidophilum gen. nov., comb. nov., in the phylum ‘Hydrogenobacter/Aquifex’. Int J Syst Evol Microbiol 51, 1853–1862.[CrossRef] [Google Scholar]
  29. Sunna, A. & Bergquist, P.(2003). A gene encoding a novel extremely thermostable 1,4-beta-xylanase isolated directly from an environmental DNA sample. Extremophiles 7, 63–70. [Google Scholar]
  30. Takai, K., Kobayashi, H., Nealson, K. H. & Horikoshi, K.(2003a).Sulfurihydrogenibium subterraneum gen. nov., sp. nov., from a subsurface hot aquifer. Int J Syst Evol Microbiol 53, 823–827.[CrossRef] [Google Scholar]
  31. Takai, K., Nakagawa, S., Sako, Y. & Horikoshi, K.(2003b).Balnearium lithotrophicum gen. nov., sp. nov., a novel thermophilic, strictly anaerobic, hydrogen-oxidizing chemolithoautotroph isolated from a black smoker chimney in the Suiyo Seamount hydrothermal system. Int J Syst Evol Microbiol 53, 1947–1954.[CrossRef] [Google Scholar]
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vol. , part 2, pp. 398 - 403

Effect of temperature on the growth of cells of strain CP.B2 . [PDF](18 KB)



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