1887

Abstract

A Gram-positive, rod-shaped, non-spore-forming bacterium (strain K362) was isolated from a deep-water marine sponge collected off the coast of Curaçao in the Netherlands Antilles. On the basis of 16S rRNA gene sequence similarities, strain K362 was shown to belong to the genus , being most closely related to (99.2 %), (98.9 %), (98.8 %), (98.8 %) and (98.8 %). A combination of the substrate utilization patterns, the fatty acid and mycolic acid profiles and the DNA–DNA hybridization results supported the affiliation of strain K362 to the genus and enabled the genotypic and phenotypic differentiation of strain K362 from the seven recognized species. Strain K362 therefore represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is K362 (=DSM 44990=NRRL B-24467).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64837-0
2007-07-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/7/1478.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64837-0&mimeType=html&fmt=ahah

References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Sideman J. G., Smith J. A., Struhl K. 1987 Current Protocols in Molecular Biology New York: Wiley;
    [Google Scholar]
  2. Boiron P., Provost F., Duport B. 1993 Laboratory Methods for the Diagnosis of Nocardiosis pp  107–126 Paris: Institut Pasteur;
    [Google Scholar]
  3. Collins M. D., Smida J., Dorsch M., Stackebrandt E. 1988; Tsukamurella gen. nov., harboring Corynebacterium paurometabolum and Rhodococcus aurantiacus . Int J Syst Bacteriol 38:385–391 [CrossRef]
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurements of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  5. Gillis M., De Ley J., De Cleene M. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur J Biochem 12:143–153 [CrossRef]
    [Google Scholar]
  6. Johnson J. L. 1985; DNA reassociation and RNA hybridization of bacterial nucleic acids. Methods Microbiol 18:33–74
    [Google Scholar]
  7. Kattar M. M., Cookson B. T., Carlson L. D., Stiglich S. K., Schwartz M. A., Nguyen T. T., Daza R., Wallis C. K., Yarfitz S. L., Coyle M. B. 2001; Tsukamurella strandjordae sp. nov., a proposed new species causing sepsis. J Clin Microbiol 39:1467–1476 [CrossRef]
    [Google Scholar]
  8. Nam S.-W., Chun J., Kim S., Kim W., Zakrzewska-Czerwinska J., Goodfellow M. 2003; Tsukamurella spumae sp. nov., a novel actinomycete associated with foaming in activated sludge plants. Syst Appl Microbiol 26:367–375 [CrossRef]
    [Google Scholar]
  9. Nam S.-W., Kim W., Chun J., Goodfellow M. 2004; Tsukamurella pseudospumae sp. nov., a novel actinomycete isolated from activated sludge foam. Int J Syst Evol Microbiol 54:1209–1212 [CrossRef]
    [Google Scholar]
  10. Olson J. B., Lord C. C., McCarthy P. J. 2000; Improved recoverability of microbial colonies from marine sponge samples. Microb Ecol 40:139–147
    [Google Scholar]
  11. Olson J. B., Harmody D. K., McCarthy P. J. 2002; Alpha-proteobacteria cultivated from marine sponges display branching rod morphology. FEMS Microbiol Lett 211:169–173
    [Google Scholar]
  12. Panicker G., Vickery M. C., Bej A. K. 2004; Multiplex PCR detection of clinical and environmental strains of Vibrio vulnificus in shellfish. Can J Microbiol 50:911–922 [CrossRef]
    [Google Scholar]
  13. Pikuta E. V., Marsic D., Bej A., Tang J., Krader P., Hoover R. B. 2005; Carnobacterium pleistocenium sp. nov., a novel psychrotolerant, facultative anaerobe isolated from permafrost of the Fox Tunnel in Alaska. Int J Syst Evol Microbiol 55:473–478 [CrossRef]
    [Google Scholar]
  14. Sieburth J. M. 1979 In Sea Microbes , p. 125 Oxford University Press; New York:
    [Google Scholar]
  15. Xu H.-X., Kawamura Y., Li N., Zhao L., Li T.-M., Li Z.-Y., Shu S., Ezaki T. 2000; A rapid method for determining the G+C content of bacterial chromosomes by monitoring fluorescence intensity during DNA denaturation in a capillary tube. Int J Syst Evol Microbiol 50:1463–1469 [CrossRef]
    [Google Scholar]
  16. Yassin A. F., Rainey F. A., Brzezinka H., Burghardt J., Lee H. J., Schaal K. P. 1995; Tsukamurella inchonensis sp. nov. Int J Syst Bacteriol 45:522–527 [CrossRef]
    [Google Scholar]
  17. Yassin A. F., Rainey F. A., Brzezinka H., Burghardt J., Rifai M., Seifert P., Feldmann K., Schaal K. P. 1996; Tsukamurella pulmonis sp. nov.. Int J Syst Bacteriol 46:429–436 [CrossRef]
    [Google Scholar]
  18. Yassin A. F., Rainey F. A., Burghardt J., Brzezinka H., Schmitt S., Seifert P., Zimmermann O., Mauch H., Gierth D. other authors 1997; Tsukamurella tyrosinosolvens sp. nov. Int J Syst Bacteriol 47:607–614 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64837-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64837-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error