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A cream-coloured bacterial strain, 5YN8-15T, was isolated from a wetland, Yongneup, in the Inje region of the Republic of Korea. The bacterium was facultatively anaerobic, Gram-negative, motile with a single polar flagellum and curved-rod-shaped. Based on 16S rRNA gene sequence analysis, strain 5YN8-15T is a member of the Betaproteobacteria. Closely related taxa were Gulbenkiania mobilis E4FC31T (94.9 % sequence similarity), Chromobacterium species (94.1–94.4 %), Aquitalea magnusonii TRO-001DR8T (93.2 %) and Aquaspirillum serpens IAM 13944T (92.5 %). All other species with validly published names analysed showed sequence similarities of below 92 %. Strain 5YN8-15T had ubiquinone 8 as the major isoprenoid quinone. The major fatty acids were summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH), C16 : 0 and C18 : 1 ω7c. The DNA G+C content was 63.0 mol%. Based on the data from the polyphasic study, strain 5YN8-15T represents a novel genus and species of the family Neisseriaceae, for which the name Paludibacterium yongneupense gen. nov., sp. nov. is proposed. The type strain is 5YN8-15T (=KACC 11601T=DSM 18731T).
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 1, pp. 190 - 194
Supplementary Fig. S1. Maximum-parsimony tree based on 16S rRNA gene sequences of strain 5YN8-15 T( Paludibacterium yongneupense gen. nov., sp. nov.) and some other members of the Betaproteobacteria . [PDF](28 KB)