1887

Abstract

A moderately halophilic and alkaliphilic bacterium, designated strain BH2, was isolated from non-saline garden soil in Saitama, Japan. Cells of strain BH2 were motile, aerobic, rod-shaped and Gram-positive and contained A1, -diaminopimelic acid-type murein. Growth occurred in 5.0–25 % (w/v) NaCl (optimum, 10–15 %, w/v), at pH 5.5–10.0 (optimum, pH 8.5–9.0) and at 20–40 °C. The predominant isoprenoid quinone was menaquinone-7. The major cellular fatty acids were ai-C, i-C, ai-C and i-C. The G+C content of the total DNA of strain BH2 was 35.1±0.4 mol% (±; =5). The phylogenetic distance from species with validly published names was less than 94.1 %. The phylogenetic and phenotypic characteristics indicated that strain BH2 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is BH2 (=JCM 14192=DSM 18494).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64830-0
2007-05-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/5/1081.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64830-0&mimeType=html&fmt=ahah

References

  1. Dussault, H. P. ( 1955; ). An improved technique for staining red-halophilic bacteria. J Bacteriol 70, 484–485.
    [Google Scholar]
  2. Echigo, A., Hino, M., Fukushima, T., Mizuki, T., Kamekura, M. & Usami, R. ( 2005; ). Endospores of halophilic bacteria of the family Bacillaceae isolated from non-saline Japanese soil may be transported by Kosa event (Asian dust storm). Saline Systems 1, 8.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Fritze, D. ( 1996; ). Bacillus haloalkaliphilus sp. nov. Int J Syst Bacteriol 46, 98–101.[CrossRef]
    [Google Scholar]
  5. Gregersen, T. ( 1978; ). Rapid method for distinction of gram-negative from gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef]
    [Google Scholar]
  6. Jeon, C. O., Lim, J. M., Lee, J. M., Xu, L. H., Jiang, C. L. & Kim, C. J. ( 2005; ). Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55, 1891–1896.[CrossRef]
    [Google Scholar]
  7. Kodaka, H., Armfield, A. Y., Lombard, G. L. & Dowell, V. R., Jr ( 1982; ). Practical procedure for demonstrating bacterial flagella. J Clin Microbiol 16, 948–952.
    [Google Scholar]
  8. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  9. Kushner, D. J. & Kamekura, M. ( 1988; ). Physiology of halophilic eubacteria. In Halophilic Bacteria, vol. I, pp. 109–140. Boca Raton, FL: CRC Press.
  10. Lenore, S. C., Arnold, E. G. & Andrew, D. E. ( 1998; ). Standard Methods for the Examination of Water and Wastewater, 20th edn. Washington, DC: American Public Health Association, the American Water Works Association and the Water Environment Federation.
  11. Lipman, D. J. & Pearson, W. R. ( 1985; ). Rapid and sensitive protein similarity searches. Science 227, 1435–1441.[CrossRef]
    [Google Scholar]
  12. Miyazaki, S., Sugawara, H., Gojobori, T. & Tateno, Y. ( 2003; ). DNA Data Bank of Japan (DDBJ) in XML. Nucleic Acids Res 31, 13–16.[CrossRef]
    [Google Scholar]
  13. Murray, P. R., Baron, E. J., Pfaller, M. A., TeNover, F. C. & Yolken, R. H. ( 1999; ). Manual of Clinical Microbiology, 7th edn. Washington DC: American Society for Microbiology.
  14. Oren, A. ( 2002; ). Halophilic Microorganisms and their Environments. Dordrecht: Kluwer Academic.
  15. Oren, A., Ventosa, A. & Grant, W. D. ( 1997; ). Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47, 233–238.[CrossRef]
    [Google Scholar]
  16. Pearson, W. R. & Lipman, D. J. ( 1988; ). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, 2444–2448.[CrossRef]
    [Google Scholar]
  17. Ren, P. G. & Zhou, P. J. ( 2005; ). Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China. Int J Syst Evol Microbiol 55, 95–99.[CrossRef]
    [Google Scholar]
  18. Romano, I., Lama, L., Nicolaus, B., Gambacorta, A. & Giordano, A. ( 2005; ). Alkalibacillus filiformis sp. nov., isolated from a mineral pool in Campania, Italy. Int J Syst Evol Microbiol 55, 2395–2399.[CrossRef]
    [Google Scholar]
  19. Saito, H. & Miura, K. ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef]
    [Google Scholar]
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Schleifer, K. H. ( 1985; ). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156.
    [Google Scholar]
  22. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  23. Schlesner, H., Lawson, P. A., Collins, M. D., Weiss, N., Wehmeyer, U., Völker, H. & Thomm, M. ( 2001; ). Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51, 425–431.
    [Google Scholar]
  24. Smibert, R. M. & Krieg, N. R. ( 1981; ). General characterization. In Manual of Methods for General Microbiology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  25. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt. Washington, DC: American Society for Microbiology.
  26. Tamaoka, J. ( 1986; ). Analysis of bacterial menaquinone mixtures by reverse-phase high-performance liquid chromatography. Methods Enzymol 123, 251–256.
    [Google Scholar]
  27. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–340.[CrossRef]
    [Google Scholar]
  28. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64830-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64830-0
Loading

Data & Media loading...

Supplements

Alignment of 16S rRNA gene sequences of members of the family . [PDF](343 KB)

PDF

Neighbour-joining tree showing the phylogenetic relationships of members of the family based on 16S rRNA gene sequences. [PDF](22 KB)

PDF

Signature bases in the 16S rRNA gene sequences of the genera , and and strain BH2 . [PDF](12 KB)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error