1887

Abstract

Two moderately halophilic strains, SL014B-69 and SL014B-62A2, were isolated from a saline soil contaminated with crude oil in Gudao in the coastal Shengli oilfield in China; the isolates were Gram-negative, rod-shaped and carried lateral flagella. Growth occurred at NaCl concentrations of 1–20 % (w/v), at temperatures of 10–42 °C and at pH 8.0–9.0. Strain SL014B-69 had C 7 (28.61 %), C cyclo 7 (27.97 %), C (19.66 %) and C 3-OH (8.87 %) as the predominant fatty acids and Q9 as the major ubiquinone, with the G+C content of genomic DNA being 64.0 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the two strains belonged to genus of in the , with the highest 16S rRNA gene sequence similarities of 96.4 % with ATCC 700597 and 96.0 % with FB2. DNA–DNA relatedness of strain SL014B-69 with strain SL014B-62A2, ATCC 700597 and DSM 9502 was 97.4, 42.9 and 36.8 %, respectively. On the basis of these data, a novel species of the genus , sp. nov., is proposed for strain SL014B-69 and SL014B-62A2. The type strain is SL014B-69 (=LMG 23610=CGMCC 1.6133).

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2007-05-01
2020-09-28
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References

  1. Berendes F., Gottschalk G., Heine-Dobbernack E., Moore E. R. B., Tindall B. J. 1996; Halomonas desiderata sp. nov., a new alkaliphilic, halotolerant and denitrifying bacterium isolated from a municipal sewage works. Syst Appl Microbiol 19:158–167 [CrossRef]
    [Google Scholar]
  2. Collins M. D., Goodfellow M., Minnikin D. E. 1980; Fatty acid isoprenoid quinine and polar lipid composition in the classification of Curtobacterium and related taxa. J Gen Microbiol 118:29–37
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  4. Eguchi M., Nishikawa T., Macdonald K. 1996; Responses to stress and nutrient availability by the marine ultramicrobacterium Sphingomonas sp. strain RB2256. Appl Environ Microbiol 62:1287–1294
    [Google Scholar]
  5. Embley T. M. 1991; The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Lett Appl Microbiol 13:171–174 [CrossRef]
    [Google Scholar]
  6. Franzmann P. D., Burton H. R., McMeekin T. A. 1987; Halomonas subglaciescola , a new species of halotolerant bacteria isolated from Antarctica. Int J Syst Bacteriol 37:27–34 [CrossRef]
    [Google Scholar]
  7. García M. T., Mellado E., Ostos J. C., Ventosa A. 2004; Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds. Int J Syst Evol Microbiol 54:1723–1728 [CrossRef]
    [Google Scholar]
  8. Hebert A. M., Vreeland R. H. 1987; Phenotypic comparison of halotolerant bacteria: Halomonas halodurans sp. nov., nom. rev., comb. nov.. Int J Syst Bacteriol 37347–350 [CrossRef]
    [Google Scholar]
  9. Heyrman J., Balcaen A., De Vos P., Swings J. 2002; Halomonas muralis sp. nov., isolated from microbial biofilms colonizing the walls and murals of the Saint-Catherine chapel (Castle Herberstein, Austria). Int J Syst Evol Microbiol 52:2049–2054 [CrossRef]
    [Google Scholar]
  10. Huß V. A. R., Festl H., Schleifer K.-H. 1983; Studies on the spectrometric determination of DNA hybridisation from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  11. Johnson J. L. 1994; Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology . pp  655–681 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  12. Kates M. 1986 Techniques of Lipidology , 2nd edn. Amsterdam: Elsevier;
    [Google Scholar]
  13. Kaye J. Z., Márquez C., Ventosa A., Baross J. A. 2004; Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov.: halophilic bacteria isolated from deep-sea hydrothermal-vent environments. Int J Syst Evol Microbiol 54:499–511 [CrossRef]
    [Google Scholar]
  14. Komagata K., Suzuki K. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–206
    [Google Scholar]
  15. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  16. Lim J.-M., Yoon J.-H., Lee J.-C., Jeon C. O., Park D.-J., Sung C., Kim C.-J. 2004; Halomonas koreensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea. Int J Syst Evol Microbiol 54:2037–2042 [CrossRef]
    [Google Scholar]
  17. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  18. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  19. Martínez-Cánovas M. J., Béjar V., Martínez-Checa F., Quesada E. 2004; Halomonas anticariensis sp. nov., from Fuente de Piedra, a saline-wetland wildfowl reserve in Málaga, southern Spain. Int J Syst Evol Microbiol 54:1329–1332 [CrossRef]
    [Google Scholar]
  20. Mormile M. R., Romine M. F., Garcia M. T., Ventosa A., Bailey T. J., Peyton B. M. 1999; Halomonas campisalis sp. nov., a denitrifying, moderately haloalkaliphilic bacterium. Syst Appl Microbiol 22:551–558 [CrossRef]
    [Google Scholar]
  21. Romanenko L. A., Schumann P., Rohde M., Mikhailov V. V., Stackebrandt E. 2002; Halomonas halocynthiae sp. nov., isolated from the marine ascidian Halocynthiae aurantium . Int J Syst Evol Microbiol 52:1767–1772 [CrossRef]
    [Google Scholar]
  22. Romano I., Giordano A., Lama L., Nicolaus B., Gambacorta A. 2005; Halomonas campaniensis sp. nov., a haloalkaliphilic bacterium isolated from a mineral pool of Campania Region, Italy. Syst Appl Microbiol 28:610–618 [CrossRef]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. Vreeland R. H., Litchfield C. D., Martin E. L., Elliot E. 1980; Halomonas elongata , a new genus and species of extremely salt-tolerant bacteria. Int J Syst Bacteriol 30:485–495 [CrossRef]
    [Google Scholar]
  26. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  27. Yoon J.-H., Choi S. H., Lee K.-C., Kho Y. H., Kang K. H., Park Y.-H. 2001; Halomonas marisflavae sp. nov., a halophilic bacterium isolated from the Yellow Sea in Korea. Int J Syst Evol Microbiol 51:1171–1177 [CrossRef]
    [Google Scholar]
  28. Yoon J.-H., Lee K.-C., Kho Y. H., Kang K. H., Kim C.-J., Park Y.-H. 2002; Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 52:123–130
    [Google Scholar]
  29. Zumft W. G. 1991; The denitrifying bacteria. In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Application , 2nd edn. pp  554–582 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
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