1887

Abstract

Two moderately halophilic strains, SL014B-69 and SL014B-62A2, were isolated from a saline soil contaminated with crude oil in Gudao in the coastal Shengli oilfield in China; the isolates were Gram-negative, rod-shaped and carried lateral flagella. Growth occurred at NaCl concentrations of 1–20 % (w/v), at temperatures of 10–42 °C and at pH 8.0–9.0. Strain SL014B-69 had C 7 (28.61 %), C cyclo 7 (27.97 %), C (19.66 %) and C 3-OH (8.87 %) as the predominant fatty acids and Q9 as the major ubiquinone, with the G+C content of genomic DNA being 64.0 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the two strains belonged to genus of in the , with the highest 16S rRNA gene sequence similarities of 96.4 % with ATCC 700597 and 96.0 % with FB2. DNA–DNA relatedness of strain SL014B-69 with strain SL014B-62A2, ATCC 700597 and DSM 9502 was 97.4, 42.9 and 36.8 %, respectively. On the basis of these data, a novel species of the genus , sp. nov., is proposed for strain SL014B-69 and SL014B-62A2. The type strain is SL014B-69 (=LMG 23610=CGMCC 1.6133).

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2007-05-01
2019-12-07
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