1887

Abstract

A Gram-positive, moderately halotolerant bacterium, designated CL-GR16, was isolated from coastal water off the east coast of Korea. The strain was strictly aerobic, rod-shaped, motile by means of peritrichous flagella and produced ellipsoidal spores. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate represented an independent lineage within rRNA group 1, showing 93.6–94.6 % similarity with respect to the genus , 94.0 % with respect to , 91.0–93.5 % with respect to , 93.2–93.3 % with respect to and 92.8–93.2 % with respect to . The optimum temperature and pH for growth were 30 °C and pH 7.5. Strain CL-GR16 was able to grow at NaCl concentrations from 0 to 14 %, with optimum growth occurring at 0–2 % NaCl. The strain lacked oxidase. The major fatty acids were anteiso-C (65.6 %), anteiso-C (11.0 %) and iso-C (9.1 %). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The predominant menaquinone was MK-7. The G+C content of the DNA was 43 mol%. On the basis of the results of the polyphasic analysis, strain CL-GR16 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is strain CL-GR16 (=KCCM 42329=DSM 18252).

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2007-07-01
2019-08-17
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A table showing whole-cell fatty acid composition of strain CL-GR16 and related species is available hereas an Acrobat PDF file.

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Two-dimensional thin-layer chromatogram of polar lipids from strain CL-GR16 . PG, Phosphatidylglycerol; DPG, diphosphatidylglycerol; GL, unidentified glycolipid.

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