1887

Abstract

Two Gram-positive, rod-shaped, moderately halophilic bacteria were isolated from a deep-sea carbonate rock at a methane cold seep in Kuroshima Knoll, Japan. These bacteria, strains IS-Hb4 and IS-Hb7, were spore-forming and non-motile. They were able to grow at temperatures as low as 9 °C and hydrostatic pressures up to 30 MPa. Based on high sequence similarity of their 16S rRNA genes to those of type strains of the genus , from 96.4 % (strain IS-Hb7 to NCIMB 9251) to 99.4 % (strain IS-Hb4 to D-8), the strains were shown to belong to this genus. DNA–DNA relatedness values of 49.5 % and 1.0–33.0 %, respectively, were determined between strains IS-Hb4 and IS-Hb7 and between these strains and other type strains. Both strains showed the major menaquinone MK7 and -orn–-Asp cell-wall peptidoglycan type. Straight-chain C, unsaturated C 7 alcohol and C 7 and cyclopropane C cyc fatty acids were predominant in both strains. The DNA G+C contents of IS-Hb4 and IS-Hb7 were respectively 43.3 and 42.1 mol%. Physiological and biochemical analyses combined with DNA–DNA hybridization results allowed us to place strains IS-Hb4 (=JCM 14154=DSM 18394) and IS-Hb7 (=JCM 14155=DSM 18393) in the genus as the respective type strains of the novel species sp. nov. and sp. nov.

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2007-06-01
2020-11-25
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