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Abstract

A Gram-positive, non-spore-forming bacterium (strain GW4-1778) was isolated from soil of the Italian island of Stromboli. 16S rRNA gene sequence similarity studies showed that strain GW4-1778 is a member of the genus , most closely related to (GenBank accession no. DQ659914; 98.6 %), (Z36930; 98.6 %), (AB092563; 98.4 %), (AY639902; 98.0 %), (Z46752; 98.0 %) and (AY639901; 97.8 %). Strain GW4-1778 could be distinguished from any other established species by sequence similarity values of less than 97.5 %. Strain GW4-1778 exhibited a quinone system with the predominant compound MK-8 (H, -cycl) (99.5 %) and traces of MK-8 (H), characteristic for the genus . The polar lipid profile of strain GW4-1778 consisted of the predominant compound diphosphatidylglycerol, moderate amounts of phosphatidylethanolamine, phosphatidylinositol, two phosphatidylinositol mannosides, a unknown polar lipid and trace amounts of two unknown lipids and the major fatty acids were C, C, C 8 and 10-methyl C. The results of DNA–DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW4-1778 from related species with 16S rRNA gene similarities of >97.5 %. Therefore, strain GW4-1778 merits species status, for which the name sp. nov. is proposed, with the type strain GW4-1778 (=CCUG 53410=CIP 109315=DSM 45049).

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2007-06-01
2021-03-07
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