1887

Abstract

A free-living diazotrophic strain, DS2, was isolated from corn rhizosphere. Polyphasic taxonomy was performed including morphological characterization, Biolog analysis, and 16S rRNA, and gene sequence analyses. 16S rRNA gene sequence analysis indicated that strain DS2 was closely related to the genus (96 % similarity). Chemotaxonomic characteristics (DNA G+C content 67.9 mol%; Q-10 quinone system; major fatty acid 18 : 17) were also similar to those of the genus . In all the analyses, including phenotypic characterization using Biolog analysis and comparison of cellular fatty acids, this isolate was found to be different from the closely related species , and . On the basis of these results, a novel species is proposed for this nitrogen-fixing strain. The name sp. nov. is suggested with the type strain DS2 (=NCCB 100108=LMG 23617).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64804-0
2007-03-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/3/620.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64804-0&mimeType=html&fmt=ahah

References

  1. Ben Dekhil, S., Cahill, M., Stackebrandt, E. & Sly, L. I. ( 1997; ). Transfer of Conglomeromonas largomobilis subsp. largomobilis to the genus Azospirillum as Azospirillum largimobile comb. nov., and elevation of Conglomeromonas largomobilis subsp. parooensis to the new type species of Conglomeromonas, Conglomeromonas parooensis sp. nov. Syst Appl Microbiol 20, 72–77.[CrossRef]
    [Google Scholar]
  2. Eckert, B., Weber, O. B., Kirchhof, G., Halbritter, A., Stoffels, M. & Hartmann, A. ( 2001; ). Azospirillum doebereinerae sp. nov., a nitrogen fixing bacterium associated with the C4-grass Miscanthus. Int J Syst Evol Microbiol 51, 17–26.
    [Google Scholar]
  3. Hill, J. E., Paccagnella, A., Law, K., Melito, P. L., Woodward, D. L., Price, L., Leung, A. H., Ng, L., Hemmingsen, S. M. & Goh, S. H. ( 2006; ). Identification of Campylobacter spp. and discrimination from Helicobacter and Arcobacter spp. by direct sequencing of PCR-amplified cpn60 sequences and comparison to cpnDB, a chaperonin reference sequence database. J Med Microbiol 55, 393–399.[CrossRef]
    [Google Scholar]
  4. Khammas, K. M., Ageron, E., Grimont, P. A. D. & Kaiser, P. ( 1989; ). Azospirillum irakense sp. nov., a nitrogen fixing bacterium associated with rice roots and rhizosphere soil. Res Microbiol 140, 679–693.
    [Google Scholar]
  5. Magalhães, F. M., Baldani, J. I., Souto, M., Kuykendall, J. R. & Dobereiner, J. ( 1983; ). A new acid tolerant Azospirillum species. Ann Acad Bras Cienc 55, 417–430.
    [Google Scholar]
  6. Mehnaz, S. & Lazarovits, G. ( 2006; ). Inoculation effects of Pseudomonas putida, Gluconacetobacter azotocaptans and Azospirillum lipoferum on corn plant growth under green house conditions. Microb Ecol 51, 326–335.[CrossRef]
    [Google Scholar]
  7. Mehnaz, S., Mirza, M. S., Huarat, J., Bally, R., Normand, P. & Malik, K. A. ( 2001; ). Isolation and 16S rRNA sequence analysis of the beneficial bacteria from the rhizosphere of rice. Can J Microbiol 47, 110–117.[CrossRef]
    [Google Scholar]
  8. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  9. Nautiyal, C. S. ( 1999; ). An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol Lett 170, 265–270.[CrossRef]
    [Google Scholar]
  10. Paisley, R. ( 1996; ). MIS Whole Cell Fatty Acid Analysis by Gas Chromatography Training Manual. Newark, DE: MIDI.
  11. Peng, G., Wang, H., Zhang, G., Hou, W., Liu, Y., Wang, E. T. & Tan, Z. ( 2006; ). Azospirillum melinis sp. nov., a group of diazotrophs isolated from tropical molasses grass. Int J Syst Evol Microbiol 56, 1263–1271.[CrossRef]
    [Google Scholar]
  12. Poly, F., Monrozier, L. J. & Bally, R. ( 2001; ). Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil. Res Microbiol 152, 95–103.[CrossRef]
    [Google Scholar]
  13. Reinhold, B., Hurek, T., Fendrik, I., Pot, B., Gillis, M., Kersters, K., Thielemans, S. & De Ley, J. ( 1987; ). Azospirillum halopraeferens sp. nov., a nitrogen fixing organism associated with roots of kallar grass (Leptochloa fusca (L.) Kunth). Int J Syst Bacteriol 37, 43–51.[CrossRef]
    [Google Scholar]
  14. Rennie, R. J. ( 1981; ). A single medium for the isolation of nitrogen fixing bacteria. Can J Microbiol 27, 8–14.[CrossRef]
    [Google Scholar]
  15. Tarrand, J. J., Kreig, N. R. & Dobereiner, J. ( 1978; ). A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov., and two species, Azospirillum lipoferum (beijerinck) comb. nov. and Azospirillum brasilense sp. nov. Can J Microbiol 24, 967–980.[CrossRef]
    [Google Scholar]
  16. Xie, C.-H. & Yokota, A. ( 2005; ). Azospirillum oryzae sp. nov., a nitrogen-fixing bacterium isolated from the roots of the rice plant Oryza sativa. Int J Syst Evol Microbiol 55, 1435–1438.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64804-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64804-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error