1887

Abstract

The taxonomic position of two actinomycete strains, LC2 and LC11, isolated from a filtration substrate made from Japanese volcanic soil, was determined using a polyphasic approach. The strains grew at temperatures from 5 to 45 °C, on media of pH between 6 and 11 and in the presence of 7 % NaCl. The major menaquinone was MK-9(H). The major fatty acid was iso-C. A phylogenetic tree based on 16S rRNA gene sequences showed that the two strains formed a distinct evolutionary lineage within the genus . On the basis of their morphological, physiological and genotypic characteristics, the isolates are proposed to represent two novel species of the genus , for which the names sp. nov. (type strain LC2 =IAM 15387 =CCTCC AB206019), and sp. nov. (type strain LC11 =IAM 15388 =CCTCC AB206020) are proposed.

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2007-08-01
2020-01-21
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References

  1. Ding, L. & Yokota, A. ( 2002; ). Phylogenetic analysis of the genus Aquaspirillum based on 16S rRNA gene sequences. FEMS Microbiol Lett 212, 165–169.[CrossRef]
    [Google Scholar]
  2. Ding, L. & Yokota, A. ( 2004; ). Proposals of Curvibacter gracilis gen. nov., sp. nov. and Herbaspirillum putei sp. nov. for bacterial strains isolated from well water and reclassification of [Pseudomonas] huttiensis, [Pseudomonas] lanceolata, [Aquaspirillum] delicatum and [Aquaspirillum] autotrophicum as Herbaspirillim huttiense comb. nov., Curvibacter lanceolatus comb. nov., Curvibacter delicatus comb. nov. and Herbaspirillum autotrophicum comb. nov. Int J Syst Evol Microbiol 54, 2223–2230.[CrossRef]
    [Google Scholar]
  3. Embley, T. M., Smida, J. & Stackebrandt, E. ( 1988; ). The phylogeny of mycolate-less wall chemotype IV actinomycetes and description of Pseudonocardiaceae fam. nov. Syst Appl Microbiol 11, 44–52.[CrossRef]
    [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package) version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  8. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  9. Huang, Y., Qi, W., Lu, Z., Liu, Z. & Goodfellow, M. ( 2001; ). Amycolatopsis rubida sp. nov., a new Amycolatopsis species from soil. Int J Syst Evol Microbiol 51, 1093–1097.[CrossRef]
    [Google Scholar]
  10. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  11. Kroppenstedt, R. M. ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics, pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  12. Lechevalier, M. P. & Lechevalier, H. A. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  13. Lechevalier, H. A. & Lechevalier, M. P. ( 1980; ). The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy (Society for Industrial Microbiology Special Publication no. 6), pp. 277–284. Edited by A. Dietz & D. W. Thayer. Arlington, VA: Society for Industrial Microbiology.
  14. Lechevalier, M. P., Prauser, H., Labeda, D. P. & Ruan, J. S. ( 1986; ). Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov. Int J Syst Bacteriol 36, 29–37.[CrossRef]
    [Google Scholar]
  15. Lee, S. D. & Hah, Y. C. ( 2001; ). Amycolatopsis albidoflavus sp. nov. Int J Syst Evol Microbiol 51, 645–650.
    [Google Scholar]
  16. Majumdar, S., Prabhagaran, S. R., Shivaji, S. & Lal, R. ( 2006; ). Reclassification of Amycolatopsis orientalis DSM 43387 as Amycolatopsis benzoatilytica sp. nov. Int J Syst Evol Microbiol 56, 199–204.[CrossRef]
    [Google Scholar]
  17. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  18. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M. ( 1980; ). Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–233.[CrossRef]
    [Google Scholar]
  19. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  22. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI Inc.
  23. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  24. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  25. Warwick, S., Bowen, T., McVeigh, H. & Embley, T. M. ( 1994; ). A phylogenetic analysis of the family Pseudonocardiaceae and the genera Actinokineospora and Saccharothrix with 16S rRNA sequences and a proposal to combine the genera Amycolata and Pseudonocardia in an emended genus Pseudonocardia. Int J Syst Bacteriol 44, 293–299.[CrossRef]
    [Google Scholar]
  26. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  27. Willems, A., Doignon, B. F., Goris, J., Coopman, R., de Lajudie, P., De Vos, P. & Gillis, M. ( 2001; ). DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51, 1315–1322.
    [Google Scholar]
  28. Yano, I., Saito, K., Fukukawa, Y. & Kusunose, M. ( 1972; ). Structural analysis of molecular species of nocardomycolic acids from Nocardia erythropolis by the combined system of gas chromatography and mass spectrometry. FEBS Lett 21, 215–219.[CrossRef]
    [Google Scholar]
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