1887

Abstract

An actinomycete, designated strain GIMV4.0001, was isolated from a forest soil sample in Vietnam. It produced white aerial mycelium and violet–blue diffusible pigment on Gause's synthetic agar. The substrate mycelium colour was not sensitive to pH. Micoscopic observations revealed that strain GIMV4.0001 produced long, straight chains of cylindrical spores, and chemotaxonomic data confirmed that it belongs to the genus Melanin was produced, but no antibacterial activity was evident against , , , or Analysis of the 16S rRNA gene sequence of strain GIMV4.0001 revealed that the highest similarity (99.4 %) was to ATCC 11062. However, the DNA–DNA relatedness between strain GIMV4.0001 and ATCC 11062 was found to be 50.3 %. Strain GIMV4.0001 could also be differentiated from ATCC 11062 and other species showing high 16S rRNA gene sequence similarity (98–99 %) based on morphological, physiological and biochemical characteristics. On the basis of its physiological and molecular properties, it is evident that strain GIMV4.0001 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GIMV4.0001 (=CCTCC M 205143=IAM 15340).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64774-0
2007-08-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/8/1770.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64774-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and PSI-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Atlas R. M. 1993 Handbook of Microbiological Media Edited by Parks L. C. Boca Raton, FL: CRC Press;
    [Google Scholar]
  3. Butte W. 1983; Rapid method for the determination of fatty acid profiles from fats and oils using trimethylsulphonium hydroxide for transesterification. J Chromatogr 261:142–145 [CrossRef]
    [Google Scholar]
  4. Cui X. L., Mao P. H., Zeng M., Li W. J., Zhang L. P., Xu L. H., Jiang C. L. 2001; Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . Int J Syst Evol Microbiol 51:357–363
    [Google Scholar]
  5. Edwards U., Rogall T., Blocker H., Emde M., Bottger E. C. 1989; Isolation and direct complete nucleotide determination of entire genes, characterization of gene coding for 16S ribosomal RNA. Nucleic Acids Res 17:7843–7853 [CrossRef]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Takeuchi N., Yamamoto H., Liu S.-L., Miura H., Matsui K., Yabuuchi E. 1988; Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells. J Clin Microbiol 26:1708–1713
    [Google Scholar]
  7. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  8. Kornerup A., Wanscher J. H. 1978 Methuen Handbook of Colour London: Eyre Methuen;
    [Google Scholar]
  9. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001 mega2: Molecular Evolutionary Genetics Analysis software Tempe, AZ: Arizona State University; http://www.megasoftware.net
    [Google Scholar]
  10. Lechevalier M. P., Lechevalier H. A. 1970; A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales pp 393–405 Edited by Prauser H. Jena: Gustav Fischer;
    [Google Scholar]
  11. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  12. Preobrazhenskaya T. P., Sveshnikova M. A., Terekhova L. P., Maximova T. S. 1983 A Guide for the Determination of Actinomycetes. Genera Streptomyces, Streptoverticillium, and Chainia Moscow: Nauka;
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Sawabe T., Makino H., Tatsumi M., Nakano K., Tajima K., Iqbal M. M., Yumoto L., Ezura Y., Christen R. 1998; Pseudomoalteromonas bacteriolytica sp. nov., a marine bacterium that is the causative agent of red spot disease of Laminaria japonica . Int J Syst Bacteriol 48:769–774 [CrossRef]
    [Google Scholar]
  15. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  16. Shirling E. B., Gottlieb D. 1968a; Cooperative description of type cultures of Streptomyces . II. Species descriptions from first study. Int J Syst Bacteriol 18:69–189 [CrossRef]
    [Google Scholar]
  17. Shirling E. B., Gottlieb D. 1968b; Cooperative description of type cultures of Streptomyces . III. Additional species descriptions from first and second studies. Int J Syst Bacteriol 18:279–392 [CrossRef]
    [Google Scholar]
  18. Shirling E. B., Gottlieb D. 1969; Cooperative description of type cultures of Streptomyces . IV. Species descriptions from the second, third and fourth studies. Int J Syst Bacteriol 19:391–512 [CrossRef]
    [Google Scholar]
  19. Shirling E. B., Gottlieb D. 1972; Cooperative description of type strains of Streptomyces . V. Additional descriptions. Int J Syst Bacteriol 22:265–394 [CrossRef]
    [Google Scholar]
  20. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  21. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . In Bergey's Manual of Systematic Bacteriology vol. 4 pp 2452–2492 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64774-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64774-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error