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Abstract

A polyphasic study was undertaken to establish the taxonomic position of an isolate, strain DS472, from soil in Kyoto, Japan. Phylogenetic analysis, based on the 16S rRNA gene sequences, revealed that this strain constitutes a new subline within the genus , with YIM 70056 and DSM 20669 as its nearest phylogenetic neighbours (98.2 and 97.8 % sequence similarity, respectively). DNA–DNA hybridization experiments revealed 36 and 29 % relatedness between the isolate and its phylogenetic relatives, and , respectively. Chemotaxonomic characteristics, including the major quinone MK-8(H), predominant fatty acids C, C 9 and 10-methyl C, the presence of cell-wall chemotype IV and mycolic acids, were consistent with the properties of members of the genus . The DNA GC content was 64.5 mol%. On the basis of both phenotypic and genotypic evidence, strain DS472 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain DS472 (=IAM 15415=CCTCC AB206088).

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2007-09-01
2019-10-19
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vol. , part 9, pp. 1956 - 1959

Characteristics that differentiate strain DS472 from the closely related species of the genus [PDF](49 KB)



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