1887

Abstract

A polyphasic study was undertaken to establish the taxonomic position of an isolate, strain DS472, from soil in Kyoto, Japan. Phylogenetic analysis, based on the 16S rRNA gene sequences, revealed that this strain constitutes a new subline within the genus , with YIM 70056 and DSM 20669 as its nearest phylogenetic neighbours (98.2 and 97.8 % sequence similarity, respectively). DNA–DNA hybridization experiments revealed 36 and 29 % relatedness between the isolate and its phylogenetic relatives, and , respectively. Chemotaxonomic characteristics, including the major quinone MK-8(H), predominant fatty acids C, C 9 and 10-methyl C, the presence of cell-wall chemotype IV and mycolic acids, were consistent with the properties of members of the genus . The DNA GC content was 64.5 mol%. On the basis of both phenotypic and genotypic evidence, strain DS472 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain DS472 (=IAM 15415=CCTCC AB206088).

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2007-09-01
2024-12-09
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References

  1. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  2. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  3. Ghosh A., Paul D., Prakash D., Mayilraj S., Jain R. K. 2006; Rhodococcus imtechensis sp. nov., a nitrophenol-degrading actinomycete. Int J Syst Evol Microbiol 56:1965–1969 [CrossRef]
    [Google Scholar]
  4. Goodfellow M. 1986; Genus Rhodococcus Zopf 1891, 28AL. In Bergey's Manual of Systematic Bacteriology vol 2 pp 1472–1481 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  5. Goodfellow M., Alderson G., Chun J. 1998; Rhodococcal systematics: problems and developments. Antonie Van Leeuwenhoek 74:1–12 [CrossRef]
    [Google Scholar]
  6. Goodfellow M., Isik K., Yates E. 1999; Actinomycete systematics: an unfinished synthesis. Nova Acta Leopold 312:47–82
    [Google Scholar]
  7. Harper J. J., Davis G. H. G. 1979; Two-dimensional thin-layer chromatography for amino acid analysis of bacterial cell walls. Int J Syst Bacteriol 29:56–58 [CrossRef]
    [Google Scholar]
  8. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  9. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  10. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  11. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  12. Mayilraj S., Krishnamurthi S., Saha P., Saini H. S. 2006; Rhodococcus kroppenstedtii sp. nov., a novel actinobacterium isolated from a cold desert of the Himalayas, India. Int J Syst Evol Microbiol 56:979–982 [CrossRef]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  14. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233 [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  17. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  18. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  19. Xie C.-H., Yokota A. 2003; Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [CrossRef]
    [Google Scholar]
  20. Yokota A., Takashima M. 2001; Cell wall. In Classification and Identification of Microorganisms pp 97–117 Edited by Suzuki K., Hiraishi A., Yokota A. Tokyo: Springer-Verlag; (in Japanese
    [Google Scholar]
  21. Yokota A., Tamura T., Nishii T., Hasegawa T. 1993 Kineococcus aurantiacus gen. nov., sp. nov., a new aerobic gram-positive, motile coccus with meso -diaminopimelic acid and arabinogalactan in the cell wall. Int J Syst Bacteriol 4352–57 [CrossRef]
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