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Volume 58, Issue 8

sp. nov., a chitinolytic bacterium isolated from cattle-farm compost Free

Abstract

A bacterial strain, designated X.bu-b, with chitin-, xylan-, cellulose- and starch-degrading activities, was isolated from compost at a cattle farm near Daejeon, Republic of Korea. The strain comprised Gram-positive, aerobic or facultatively anaerobic, non-motile, rod-shaped bacteria. On the basis of an analysis of 16S rRNA gene sequences, the phylogenetic position of X.bu-b was within the genus , and the strain exhibited relatively high sequence similarities with respect to DSM 20112 (98.1 %), DSM 20118 (98.1 %), DSM 20113 (98.0 %), DB5 (97.9 %), ATCC 25174 (97.7 %), XIL11 (97.5 %), DSM 20107 (97.4 %), DSM 20111 (97.3 %), O (97.3 %) and DSM 20109 (97.0 %). The phylogenetic distance from other species with validly published names was greater than 3 % (i.e. less than 97.0 % sequence similarity). Chemotaxonomic data also supported the classification of strain X.bu-b within the genus : -ornithine was the cell-wall diamino acid, anteiso-C and anteiso-C were the major fatty acids, rhamnose, galactose, xylose and ribose were the cell-wall sugars, MK-9(H) was the predominant menaquinone and diphosphatidylglycerol and phosphatidylglycerol were present in the polar lipids. The G+C content of the genomic DNA was 73.6 mol%. DNA–DNA hybridization experiments showed that the values for DNA–DNA relatedness between strain X.bu-b and the phylogenetically closest neighbours were below 23 %. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain X.bu-b represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is X.bu-b (=KCTC 19133 =DSM 17922).

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2008-08-01
2022-01-20
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Cellulomonas chitinilytica sp. nov., a chitinolytic bacterium isolated from cattle-farm compost
Int J Syst Evol Microbiol 58, 1878 (2008); https://doi.org/10.1099/ijs.0.64768-0
/content/journal/ijsem/10.1099/ijs.0.64768-0
/content/journal/ijsem/10.1099/ijs.0.64768-0
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vol. , part 8, pp. 1878 - 1884

Comparison of selected characteristics (obtained using API 20NE and API 32GN galleries) of sp. nov. X.bu-b with those of the type strains of the two species to which it is most closely related.

Cellular fatty acid composition (%) of sp. nov. X.bu-b and related species.

Two-dimensional thin-layer chromatogram of the polar lipids of strain X.bu-b .

[PDF file of Supplementary Tables and Figure](110 KB)

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