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A bacterial strain, designated X.bu-bT, with chitin-, xylan-, cellulose- and starch-degrading activities, was isolated from compost at a cattle farm near Daejeon, Republic of Korea. The strain comprised Gram-positive, aerobic or facultatively anaerobic, non-motile, rod-shaped bacteria. On the basis of an analysis of 16S rRNA gene sequences, the phylogenetic position of X.bu-bT was within the genus Cellulomonas, and the strain exhibited relatively high sequence similarities with respect to Cellulomonas biazotea DSM 20112T (98.1 %), C. cellasea DSM 20118T (98.1 %), C. fimi DSM 20113T (98.0 %), C. terrae DB5T (97.9 %), C. humilata ATCC 25174T (97.7 %), C. xylanilytica XIL11T (97.5 %), C. uda DSM 20107T (97.4 %), C. gelida DSM 20111T (97.3 %), C. iranensis OT (97.3 %) and C. flavigena DSM 20109T (97.0 %). The phylogenetic distance from other Cellulomonas species with validly published names was greater than 3 % (i.e. less than 97.0 % sequence similarity). Chemotaxonomic data also supported the classification of strain X.bu-bT within the genus Cellulomonas: l-ornithine was the cell-wall diamino acid, anteiso-C15 : 0 and anteiso-C17 : 0 were the major fatty acids, rhamnose, galactose, xylose and ribose were the cell-wall sugars, MK-9(H4) was the predominant menaquinone and diphosphatidylglycerol and phosphatidylglycerol were present in the polar lipids. The G+C content of the genomic DNA was 73.6 mol%. DNA–DNA hybridization experiments showed that the values for DNA–DNA relatedness between strain X.bu-bT and the phylogenetically closest neighbours were below 23 %. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain X.bu-bT represents a novel species of the genus Cellulomonas, for which the name Cellulomonas chitinilytica sp. nov. is proposed. The type strain is X.bu-bT (=KCTC 19133T =DSM 17922T).
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 8, pp. 1878 - 1884
Supplementary Table S1. Comparison of selected characteristics (obtained using API 20NE and API 32GN galleries) of Cellulomonas chitinilytica sp. nov. X.bu-b Twith those of the type strains of the two Cellulomonas species to which it is most closely related.
Supplementary Table S2. Cellular fatty acid composition (%) of C. chitinilytica sp. nov. X.bu-b Tand related Cellulomonas species.
Supplementary Fig. S1. Two-dimensional thin-layer chromatogram of the polar lipids of strain X.bu-b T.
[PDF file of Supplementary Tables and Figure](110 KB)