1887

Abstract

Among a large collection of Taiwanese soil isolates, a novel Gram-variable, rod-shaped, motile and endospore-forming bacterial strain, designated G-soil-2-3, was isolated from farmland soil in Wu-Feng, Taiwan. The isolate was subjected to a polyphasic study including 16S rRNA gene sequence analysis, DNA–DNA hybridization experiments, fatty acid analysis and comparative phenotypic characterization. 16S rRNA gene sequence analysis indicated that the organism belongs within the genus . It contained menaquinone MK-7 as the predominant isoprenoid quinone and anteiso-C (40.5 %), iso-C (13.1 %), iso-C (10.8 %) and anteiso-C (7.3 %) as the major fatty acids. Phylogenetically, the closest relatives of strain G-soil-2-3 were the type strains of , and , with 16S rRNA gene sequence similarity of 95.7, 95 and 95.2 %, respectively. DNA–DNA hybridization experiments showed levels of relatedness of 2.8–9.0 % of strain G-soil-2-3 with these strains. The G+C content of the DNA was 44.6 mol%. Strain G-soil-2-3 was clearly distinguishable from , and and thus represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is G-soil-2-3 (=BCRC 17411=IAM 15414=LMG 23799=DSM 18679).

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2007-06-01
2019-10-14
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