1887

Abstract

The taxonomic position of sp. B13, isolated as a 3-chlorobenzoate-degrading organism and used for several groundbreaking studies on the enzymology and genetics of the degradative pathway for haloaromatic compounds, was studied in detail. The previously performed physiological studies, the detection of ubiquinone Q-9, the polyamine pattern with putrescine and spermidine as major polyamines, a fatty acid profile with C 7, summed feature 3 and C as quantitatively the most important constituents and the 16S rRNA gene sequence demonstrated that sp. B13 indeed belongs to the genus . The sequence of the sp. B13 16S rRNA gene demonstrated a high degree of similarity with that of DSM 50332 (98.9 %), DSM 14399 (98.7 %), DSM 16612 (98.1 %) and DSM 17553 (98.7 %). Thus it was shown that strain sp. B13 can be distinguished from related species by the ability/inability to assimilate -acetylgalactosamine, -galactose, putrescine, -aconitate and mesaconate and some differences in the fatty acid profile. The positioning of sp. B13 as a separate taxon was finally verified by DNA hybridization, which demonstrated less than 45 % DNA–DNA similarity between strain sp. B13 and the reference strains. On the basis of these results, sp. B13 represents a novel species for which the name sp. nov. is proposed. The type strain is B13 (=DSM 6978=LMG 23759).

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2007-03-01
2020-01-22
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