1887

Abstract

A rod-shaped, phototrophic, purple sulfur bacterium, strain JA124, was isolated in pure culture from a marine aquaculture pond, located near Bhimunipatnam, in a medium that contained 3 % NaCl (w/v). Strain JA124 is a Gram-negative, motile rod with a single polar flagellum. Strain JA124 has a requirement for NaCl, with optimum growth at 1.5–8.5 %, and tolerates up to 11 % NaCl. Intracellular photosynthetic membranes are of the vesicular type. Bacteriochlorophyll and probably carotenoids of the spirilloxanthin series are present as photosynthetic pigments. Strain JA124 was able to utilize sulfide, sulfate, thiosulfate, sulfite, thioglycollate and cysteine as sulfur sources. Strain JA124 was able to grow photolithoautotrophically, photolithoheterotrophically and photo-organoheterotrophically. Chemotrophic and fermentative growth could not be demonstrated. Strain JA124 lacks diazotrophic growth and acetylene reduction activity. Pyridoxal phosphate is required for growth. During growth on reduced sulfur sources as electron donors, sulfur is deposited intermediately as a number of small granules within the cell. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain JA124 clusters with species of the genus belonging to the class . The highest sequence similarities of strain JA124 were found with the type strains of (98 %), (95 %) and (93 %). However, DNA–DNA hybridization with DSM 15907 revealed relatedness of only 65 % with strain JA124. The DNA base composition of strain JA124 was 67 mol% G+C (by HPLC). Based on 16S rRNA gene sequence analysis, morphological and physiological characteristics and DNA–DNA hybridization studies, strain JA124 (=ATCC BAA-1316=JCM 13911) is sufficiently different from other species to merit its description as the type strain of a novel species, sp. nov.

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2007-06-01
2019-12-09
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Phase-contrast micrograph of cells of strain JA124 . Bar, 2 µm.

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Electron micrograph of ultrathin section of a cell of strain JA124 showing the vesicular nature of photosynthetic membranes extending throughout the cell (denoted by arrows). Bar, 0.5 µm.

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Whole-cell absorption spectrum of strain JA124 (a) and spectrum of acetone-extracted pigments (b).

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